滇西18种豆科植物体外降脂活性研究

In vitro Lipid-Lowering Activity of 18 Leguminosae Plants in West Yunnan

目的:研究滇西18种豆科植物的体外降脂活性,为发现植物来源的新型降脂天然产物奠定基础。方法:利用75%乙醇对18种豆科植物进行热回流提取;采用高脂HepG2细胞模型对各植物提取物进行体外降脂活性筛选,通过降脂率对其活性进行评价。结果:13种植物提取物具有不同程度的降脂活性,其中云南甘草地上部分、截叶铁扫帚全株、黑毛多枝黄芪地下部分、鞍叶羊蹄甲地上部分提取物的降脂活性较好,终浓度为0.5 mg/mL时的降脂率分别为(21.6±3.9)%、(19.6±10.0)%、(17.8±1.9)%、(15.3±4.6)%。结论:云南甘草地上部分、截叶铁扫帚全株、黑毛多枝黄芪地下部分、鞍叶羊蹄甲地上部分具有较好的降脂活性,值得后续深入研究。

Objective:To explore the in vitro lipid-lowering activity of 18 Leguminosae plants in west Yunnan,and to provide a scientific basis for the discovery of novel hypolipidemic drugs from plants.Methods:18 Leguminosae plants were extracted with 75%ethanol by heat reflux.High-fat HepG2 cell model was used to screen the lipid-lowering activity of the crude extracts in vitro,and the bioactive results were evaluated by lipid-lowering rates.Results:13 plant extracts showed lipid-lowering activity in different degrees.Among them,the aerial parts of Glycyrrhiza yunnanensis,the whole herb of Lespedeza cuneata,the underground parts of Astragalus polycladus var.nigrescens and the aerial parts of Bauhinia brachycarpa,showed significant activities to inhibit lipid accumulation,with inhibition ratios of(21.6±3.9)%,(19.6±10.0)%,(17.8±1.9)%and(15.3±4.6)%,respectively,at concentration of 0.5 mg/mL.Conclusion:The aerial parts of Glycyrrhiza yunnanensis,the whole herb of Lespedeza cuneata,the underground parts of Astragalus polycladus var.nigrescens and the aerial parts of Bauhinia brachycarpa,have good lipid-lowering activity,which is worth a further study.