美洲大蠊提取物对海马神经元细胞HT22凋亡的影响

Effects of Periplaneta americana Extracts on Apoptosis of Hippocampal Neuronal Cell HT22

目的:研究不同的美洲大蠊提取物对体外培养的小鼠海马神经元细胞系HT22细胞凋亡的影响。方法:制备美洲大蠊水提物、美洲大蠊40%、60%及95%醇提物、美洲大蠊粗提物等5种提取物,对体外培养的HT22细胞进行干预,设置溶剂对照组及浓度为60、80、100、120μg/mL的美洲大蠊提取物组,用流式细胞仪检测美洲大蠊提取物对HT22细胞凋亡的影响。结果:与溶剂对照比较,美洲大蠊粗提物组和95%醇提物组有促HT22细胞凋亡的作用,差异有统计学意义(P<0.05);美洲大蠊水提物组和40%醇提物组有抗HT22细胞凋亡的作用,差异有统计学意义(P<0.05);美洲大蠊60%醇提物组对细胞凋亡无明显影响,差异无统计学意义(P>0.05)。结论:美洲大蠊不同提取物对HT22小鼠海马神经元的凋亡影响不同。

Objective:To observe the effects of components extracted from Periplaneta americana with different solvents on the apoptosis of mouse hippocampal neuronal cell lines HT22 cells cultured in vitro.Methods:Water extract,40%alcohol extract,60%alcohol extract,95%alcohol extract and crude extract of Periplaneta americana were prepared.And the above five components were used to intervene in the HT22 cells cultured in vitro.The solvent control group and Periplaneta americana extract groups with final concentrations of 60,80,100 and 120μg/mL were set for each component.Flow cytometry was used to detect the effect of each component of Periplaneta americana extract on HT22 cell apoptosis.Results:Compared with the solvent control,the crude extract and the 95%alcohol extract of Periplaneta americana promoted the apoptosis of HT22 cells,and the differences were statistically significant(P<0.05);the water extract and the 40%alcohol extract of Periplaneta americana inhibited apoptosis of HT22 cells,and the differences were statistically significant(P<0.05);60%ethanol extract of Periplaneta americana has no obvious effect on cell apoptosis,the differences were not statistically significant(P>0.05).Conclusion:The different components of Periplaneta americana extract have different effects on the apoptosis process of mouse hippocampal neurons.