系统性红斑狼疮中肠道菌群与低氧诱导因子-1α表达水平的相关性研究

Correlation between Intestinal Flora and Expression of Hypoxia Inducible Factor-1αin Systemic Lupus Erythematosus

目的探讨SLE肠道菌群以及低氧诱导因子-1α与疾病活动度之间的关系。方法选取于本院就诊的90例SLE患者,加上于本院门诊体检的30例健康志愿者体检数据,分为A、B、C、D组,其中A组为初诊SLE患者共计30例,B组为初诊SLE给药治疗后的患者共计30例,C组为SLE复诊患者共计30例,D组为健康志愿者共计30例,将该4组的粪便、血液等标本数据进行收集和分析研究。结果与其他三组相比较,A组患者粪便中双歧杆菌、乳酸杆菌数以及大肠埃希菌数减少(P<0.05),HIF-1a显著增高(P<0.05),B细胞显著性增高(P<0.05),T细胞显著性降低(P<0.05);与其他两组SLE患者对比,B组患者粪便中双歧杆菌、乳酸杆菌数以及大肠埃希菌数显著性增高(P<0.05),HIF-1a显著降低(P<0.05),B细胞显著性降低(P<0.05),T细胞显著性升高(P<0.05),但肠道双歧杆菌、乳酸杆菌数仍低于D组(P<0.05),且血液中HIF-1a水平,B细胞个数均高于D组(P<0.05),T细胞个数均低于D组(P<0.05)。SLE患者SLEDAI评分降低,低氧诱导因子-1α水平下降、但仍高于D组。结论SLE患者存在较明显的肠道菌群失调状态。临床相关治疗手段可能会进一步加重肠道菌群失调状态。双歧杆菌、低氧诱导因子-1α与B、T淋巴细胞在SLE的发病及治疗作用机制占据极为特殊的位置,调节肠道菌群有利于改善SLE中低氧诱导因子-1α与B、T淋巴细胞的水平,对SLE的治疗预后有明显的作用。

Objective To investigate the relationship between SLE intestinal flora and hypoxia-inducible factor-1αand disease activity.Methods Data of 90 SLE patients and 30 healthy volunteers were collected and analyzed.They were divided into groups A,B,C,and D,group A had a total of 30 patients with newly diagnosed SLE,group B had a total of 30 patients with newly diagnosed SLE and medicine treatment,group C had a total of 30 patients with revisited SLE,and group D had a total of 30 healthy volunteers.The data of feces and blood samples were collected and analyzed.Results Compared with the other three groups,the number of Bifidobacterium,Lactobacillus and Escherichia coli in the stool of patients in group A decreased(P<0.05),HIF-1a increased significantly(P<0.05),and B cells significantly increased(p<0.05),T cell significantly decreased(P<0.05).Compared with the other two groups of SLE patients,the number of Bifidobacterium,Lactobacillus and Escherichia coli in the feces of group B patients increased significantly(P<0.05),HIF-1a significantly decreased(P<0.05),B cells significantly decreased(P<0.05),T cells significantly increased(P<0.05),but the number of intestinal bifidobacteria and lactobacilli was still lower than that of group D(P<0.05),and the level of HIF-1a in the blood,the number of B cells was higher than that of group D(P<0.05),and the number of T cells was lower than that of group D(P<0.05).The SLEDAI score of SLE patients decreased,and the level of hypoxia-inducible factor-1αdecreased,but it was still higher than that of group D.Conclusion SLE patients have a more obvious intestinal flora imbalance state.Clinical treatment may further aggravate the dysbacteriosis of intestinal flora.The bifidobacterium,hypoxia inducible factor-1αand B、T lymphocytes occupy a very special position in the pathogenesis and therapeutic mechanism of SLE.Regulating intestinal flora is beneficial to improve the level of SLE low oxygen inducible factor-1 and B、T lymphocytes,and has a significant effect on the therapeutic prognosis of SLE.