豨莶草提取物对冠状动脉结扎大鼠心肌AKT及ERK1/2表达的影响

Effects of Siegesbeckia orientalis extract on the expression of AKT and ERK1/2 in myocardium of rats with coronary artery ligation

[目的]通过豨莶草提取物对心肌缺血大鼠模型心肌蛋白激酶(AKT)及丝裂原活化蛋白激酶1/2(ERK1/2)表达的影响,探讨其对大鼠心肌缺血性损伤的保护作用。[方法]将90只SD大鼠雄性随机分为6组,每组15只,分别为假手术组、模型对照组、豨莶草提取物低剂量组、豨莶草提取物中剂量组、豨莶草提取物高剂量组、硝酸异山梨酯组。构建大鼠心肌缺血损伤模型,用无创缝合线在左心耳根部下方2 mm处结扎。连续给药4周后。大鼠腹主动脉采血检测心肌酶,采用苏木精-伊红(HE)染色法观察缺血区心肌损伤级别,采用免疫组化法检测心肌细胞AKT及ERK1/2的表达。[结果]与模型对照组相比较,豨莶草提取物低剂量组、中剂量组和高剂量组血清谷草转氨酶(AST)显著降低;豨莶草提取物低剂量组、中剂量组和高剂量组血清乳酸脱氢酶(LDH)有所降低,但未见显著性差异;豨莶草提取物高剂量组血清肌酸激酶(CK)显著降低;豨莶草提取物中剂量组、高剂量组血清肌酸磷酸激酶(CK-MB)显著降低;豨莶草提取物低剂量组、中剂量组、高剂量组缺血区心肌组织HE染色分级显著降低;豨莶草提取物低剂量组、中剂量组、高剂量组缺血区心肌细胞AKT的表达显著增加;豨莶草提取物中剂量组、高剂量组缺血区心肌细胞ERK1/2表达显著增加。[结论]豨莶草提取物可通过增加心肌缺血区AKT、ERK1/2表达而产生对心肌损伤的保护作用。

[Objective]Through the effect of Siegesbeckia orientalis extract on the expression of myocardial AKT and ERK1/2 in myocardial ischemic rat model,to explore its protective effect on myocardial ischemic injury in rats.[Methods]The 90 male SD rats were randomly divided into 6 groups,each with 15 rats,namely sham operation group,model control group,Siegesbeckia orientalis extract low-dose group,Siegesbeckia orientalis extract middle-dose group,Siegesbeckia orientalis extract high-dose group,isosorbide dinitrate group.The rat model of myocardial ischemia injury was constructed and ligated with 2 mm below the root of the left atrial appendage.After 4 weeks of continuous administration,blood was collected from the abdominal aorta of the rats to detect myocardial enzymes.The level of myocardial damage in the ischemic area was observed by HE staining.The expression of AKT and ERK1/2 in myocardial cells was detected by immunohistochemistry.[Results]Compared with the model control group,serum AST of Siegesbeckia orientalis extract low-dose group,middle-dose group and high-dose group were significantly reduced.Serum LDH of Siegesbeckia orientalis extract low-dose group,middle-dose group and high-dose group reduced,but no significant difference.Serum CK significantly decreased in the high-dose Siegesbeckia orientalis extract group;Serum CK-MB in the Siegesbeckia orientalis extract middle-dose group and high-dose group significantly decreased.The HE staining grade of myocardial tissue in the ischemic area of Siegesbeckia orientalis extracts in the low-dose,middle-dose and high-dose groups was significantly reduced.The expression of AKT in myocardial cells in the ischemic area of Siegesbeckia orientalis extract increased significantly in the low-dose,middle-dose and high-dose groups;the expression of ERK1 in myocardial cells in the ischemic area of Siegesbeckia orientalis extract increased significantly in the middle-dose group and high-dose group.[Conclusion]Siegesbeckia orientalis extract can increase the expression of AKT and ERK1 in the ischemic area of myocardium to produce a protective effect on myocardial injury.