QPRT、miR-26b-5p在乳腺癌中表达及其与患者预后的关系

The expression of QPRT and miR-26b-5p in breast cancer and their relationship with the prognosis of patients

目的分析喹啉酸磷酸核糖基转移酶(QPRT)、微小RNA-26b-5p(miR-26b-5p)在乳腺癌中表达及其与患者预后的关系。方法选取2015年3月—2017年10月于广州市中西医结合医院行乳腺癌(经病理组织检查均为乳腺浸润性导管癌)改良根治术女性患者89例为研究对象,获取乳腺癌组织(观察组)和癌旁正常乳腺组织(对照组),免疫组化法检测QPRT表达,原位杂交技术检测miR-26b-5p表达水平,分析QPRT、miR-26b-5p水平与患者术后复发转移的关系,COX多因素分析乳腺癌患者发生复发转移的影响因素。结果观察组QPRT的阳性表达率明显高于对照组,miR-26b-5p的阳性表达率明显低于对照组(χ^(2)/P=54.395/0.000、23.448/0.000);QPRT与miR-26b-5p在乳腺癌组织中表达呈负相关(r/P=-0.343/0.000);QPRT、miR-26b-5p表达水平与患者TNM分期、肿瘤分化程度、淋巴结转移有关(QPRT:χ^(2)/P=5.326/0.021、4.991/0.025、6.740/0.009,miR-26b-5p:χ^(2)/P=5.902/0.015、4.946/0.026、7.949/0.005);QPRT高表达患者术后复发转移率明显高于QPRT低表达患者(65.38%vs.29.73%,χ^(2)/P=13.260/0.000),miR-26b-5p低表达患者术后复发转移率明显高于miR-26b-5p高表达患者(63.79%vs.25.81%,χ^(2)/P=12.550/0.000);分化程度低、TNM分期Ⅲ~Ⅳ期、淋巴结发生转移、QPRT高表达、miR-26b-5p低表达是影响乳腺癌患者术后复发转移的危险因素[OR(95%CI)=2.381(1.394~4.066)、2.317(1.272~4.221)、2.449(1.243~4.825)、2.604(1.501~4.517)、3.007(1.625~5.564)]。结论乳腺癌组织中QPRT表达上调,miR-26b-5p表达下调,是影响乳腺癌患者术后发生复发转移的危险因素,可能为临床治疗乳腺癌提供潜在靶点。

Objective To analyze the expression of quinolinic acid phosphoribosyl transferase(QPRT)and microRNA-26b-5p(miR-26b-5p)in breast cancer and their relationship with the prognosis of patients.Methods From March 2015 to October 2017,89 female patients undergoing modified radical mastectomy in the Breast Department of Guangzhou Hospital of Integrated Traditional Chinese and Western Medicine(all were invasive ductal carcinoma of the breast after pathological examination)were selected as the research objects,and breast cancer tissues were obtained.(Observation group)and normal breast tissue adjacent to cancer(control group),immunohistochemical method was used to detect QPRT expression,in situ hybridization was used to detect miR-26b-5p expression level,and the relationship between QPRT,miR-26b-5p level and postoperative recurrence and metastasis was analyzed.Factor analysis of the influencing factors of recurrence and metastasis of breast cancer patients.Results The positive expression rate of QPRT in the observation group was significantly higher than that of the control group,and the positive expression rate of miR-26b-5p was significantly lower than that of the control group(χ^(2)/P=54.395/0.000,23.448/0.000);QPRT and miR-26b-5p were found in breast cancer tissues The expression was negatively correlated(r/P=-0.343/0.000).The expression levels of QPRT and miR-26b-5p were related to the patient’s TNM stage,tumor differentiation,and lymph node metastasis(QPRT:χ^(2)/P=5.326/0.021,4.991/0.025,6.740/0.009,miR-26b-5p:χ^(2)/P=5.902/0.015,4.946/0.026,7.949/0.005);the postoperative recurrence and metastasis rate of patients with high QPRT expression was significantly higher than that of patients with low QPRT expression(65.38%vs.29.73%,χ^(2)/P=13.260/0.000),the postoperative recurrence and metastasis rate of patients with low miR-26b-5p expression was significantly higher than that of patients with high miR-26b-5p expression(63.79%vs.25.81%,χ^(2)/P=12.550/0.000);the degree of differentiation was low,TNM stage III-IV,Lymph node metastasis,high QPRT expression,and low miR-26b-5p expression are risk factors affecting postoperative recurrence and metastasis of breast cancer patients[OR(95%CI)=2.381(1.394-4.066),2.317(1.272-4.221),2.449(1.243-4.825),2.604(1.501-4.517),3.007(1.625-5.564)].Conclusion The up-regulation of QPRT expression in breast cancer tissues and the down-regulation of miR-26b-5p expression are risk factors affecting postoperative recurrence and metastasis of breast cancer patients,and may provide potential targets for clinical treatment of breast cancer.