激光散斑成像仪测量结果的影响因素

Effect factors of the measurement results of Laser Speckle Imaging Analyzer

目的通过模拟不同的使用条件,找出影响激光散斑衬比成像(LSCI)结果值的影响因素。方法在暨南大学第一附属医院的疼痛门诊模拟各种使用条件。采用武汉迅微光电子技术有限公司的商用仪器(Laser Speckle Blood Flow ImagerⅡ),利用仿皮肤硅胶模型作为被测物体,探索4种使用条件下散斑成像系统结果值的变化:包括肤色、绿、黄、蓝、红、黑6种物体颜色的不同的曝光时间(1~15 ms)、不同的光线条件(遮光、室内灯光及无额外光照)、不同的探头距被测物体距离(15、20、25、30、35、40、45、50 cm)以及不同的探头激光束与被测物体夹角(90°、85°、80°、75°、70°、65°)。结果曝光时间影响:6种被测物体颜色的散斑成像测量结果值都会随着曝光时间的增加而减少;仅当被测物体表面是黑色时,测量结果值减少的变化规律和其他5种颜色的变化规律不一致。光线条件影响:被测物体表面颜色为红色时,3种光线条件下的散斑成像测量结果值差异无统计学意义;当被测物体表面颜色为黑色时,仅室内灯光和无光条件下的散斑成像测量结果值差异无统计学意义。探头距被测物体距离的影响:被测物体表面颜色为红色时,在20~40 cm探头距被测物体距离范围内,散斑成像测量结果值差异无统计学意义;当被测物体表面颜色为黑色时,在20~35 cm探头距被测物体距离范围内,散斑成像测量结果值无明显统计学差异。探头激光束与被测物体夹角的影响:被测物体表面颜色为红色,曝光时间为5 ms时,6个探头激光束与被测物体夹角下的散斑成像测量结果值差异均无统计学意义;曝光时间为10 ms时,在80°~90°和65°~85°两个探头激光束与被测物体夹角范围内,散斑成像测量结果值差异无统计学意义。当被测物体表面颜色为黑色,曝光时间为5 ms、角度在80°~90°及65°~75°范围内时,散斑成像测量结果值差异无统计学意义;曝光时间为10 ms、角度在85°~90°及65°~80°范围内时,散斑成像测量结果值差异无统计学意义。结论曝光时间、光线条件、探头距被测物体距离及激光束与被测物体夹角均是影响激光散斑成像仪测量结果值的影响因素。除此之外,被测物体表面颜色是黑色时的测量结果值和其他任何颜色都不一致,我们应该重新审视不同被测者的测量结果值的可比性。

Objective To find out the factors that affect the measurement results of laser speckle contrast imaging(LSCI)by simulating using conditions.Methods We simulated using conditions in the pain clinic of The First Affiliated Hospital of Jinan University.The LSCI device we used is a commercial product from SIM Opto-Technology Co.,Wuhan,China(Laser Speckle Blood Flow ImagerⅡ).Using a silicon-imitated skin model,we collected the results changes of Laser Speckle Blood Flow ImagerⅡdata in four using conditions,including different exposure times(1-15 ms),different lighting situations(shading condition,lamplight condition,and no light condition),different distances between the LSCI device and object(15,20,25,30,35,40,45,50 cm),and different angles between the LSCI device laser beam and object(90°,85°,80°,75°,70°,65°).Results The influence of the exposure time:The measurement results of the six colors area of the object decreased when the setting of exposure time increased,and particularly showed different change pattern when the object color was black,compared to the other five colors.The influence of the light condition:When the detected area was red,the laser speckle results showed no significant difference in three light conditions;when the detected area was black,the laser speckle results showed no significant difference only in lamplight conditions and no light conditions.The influence of the distance of the device and object:When the detected area was red,and the distances were between 20-40 cm,the laser speckle results showed no significant difference;when the detected area was black,and the distances were between 20-35 cm,the laser speckle results showed no significant difference.The influence of the angle between the device laser beam and the object:When the detected area was red,and the exposure time was 5 ms,the laser speckle results showed no significant difference in all six angles between the device laser beam and object;and when the exposure time was 10ms,the laser speckle results showed no significant difference in two ranges of 80°-90°and 65°-85°.When the detected area was black,and the exposure time was 5 ms,the laser speckle results showed no significant difference in two ranges of 80°-90°and 65°-75°;when the exposure time was 10 ms,the laser speckle results showed no significant difference in two ranges of 85°-90°and 65°-80°.Conclusion Exposure time,lighting condition,the distance between the LSCI device and object,the angle between the device laser beam and the object are all factors that affect the measurement results.In addition,compared to other colors,measurement results varied from the other results when the color of object is black,we should reconsider the comparability of LSCI blood flow value between different people.