侵染番茄的曲叶病毒检测及遗传变异分析

Detection of Leaf Curl Virus Infected Tomato and Genetic Evolution Analysis

2017—2019年,从甘肃、广西、海南、河北和云南5个省份番茄主产区采集疑似感染曲叶病毒的番茄病叶,进行曲叶病毒检出率分析及种类鉴定。特异性PCR检测结果表明,5个省份曲叶病毒平均检出率为25.00%,其中广西最高,达到50.91%。PCR扩增序列同源性比对结果表明,侵染5个省份番茄的曲叶病毒为中国番木瓜曲叶病毒(papaya leaf curl China virus,PaLCuCNV)、广东番茄曲叶病毒(tomato leaf curl Guangdong virus,ToLCGDV)和广西番茄曲叶病毒(tomato leaf curl Guangxi virus,ToLCGXV)。序列系统发育分析表明,5个省份的曲叶病毒分离物可分为2个亚簇,无明显的地域特性和寄主依赖性。遗传进化分析表明,5个省份的曲叶病毒出现5个重组事件,序列中C/T替换率最高(18.73)。

From 2017-2019,this experiment collected tomato samples suspected infecting with leaf curl viruses from Gansu,Guangxi,Hainan,Hebei and Yunnan Provinces and conducted analysis and identification on leaf curl viruses species.The results of detection by specific PCR showed that the mean value of positive ratio of tomato samples infected with leaf curl viruses was 25.00%,among them samples from Guangxi was the highest,reaching 50.91%.The results of identity analysis of PCR amplified sequences indicated that infecting 5 provinces’tomato leaf curl viruses were papaya leaf curl China virus(PaLCuCNV),tomato leaf curl Guangdong virus(ToLCGDV),and tomato leaf curl Guangxi virus(ToLCGXV).The sequence systematic development phylogenetic analysis revealed that the leaf curl viruses from 5 provinces could be divided into 2 sub-clusters without distinguish regional character and host dependent.Genetic evolution analysis showed that there were 5 recombinant events existed in leaf curl viruses from these 5 provinces,and the highest substitution ratio of 18.73 was existed in C/T.