口腔未培养微生物分离培养策略的研究进展

Research strategies on isolation and cultivation of uncultivated oral microorganisms

口腔微生物是人体微生物组的重要组成部分,其群落组成丰富且独特。现有研究显示,口腔微生物与龋病、牙周炎等口腔健康问题有直接的联系,因而具有重要的研究价值。随着高通量测序技术的发展,人们对口腔中未培养微生物多样性的认识不断加深,这进一步催生对微生物分离培养技术需求的增加。为此,本文将围绕口腔未培养微生物及其分离培养策略的研究进展,首先介绍口腔中未培养微生物的研究现状;其次分析口腔微生物分离培养中可能的限制因素;最后综述微生物分离培养技术发展及其在口腔未培养微生物研究中的应用。全文旨在为口腔未培养微生物的分离培养提供思路和技术参考。

Representing a fundamental part of the human microbiota,the oral microbial community is characterized by its diverse and unique composition.Oral diseases like dental caries and periodontitis are directly associated with oral microorganisms.Therefore,it is crucial to deepen our understanding of oral microbiota.Advances in high-throughput sequencing technology provide extensive information of the diversity of oral uncultivated microorganisms,prompting an increasing need for microbial isolation and cultivation techniques.This review presents recent research progress on uncultivated oral microorganisms and lists factors that possibly hinder isolation attempts.In addition,advances in methodologies and techniques used for culturing previously uncultured microorganisms and their applications in oral microbiology studies are summarized,giving valuable insights into various aspects of uncultivated oral microorganisms.