杂交鲌“先锋1号”染色体组型分析和DNA含量测定

Karyotype and Cellular DNA Contents of Culter Hybrid F_(1) Erythroculter ilishaeformis(♀)×Ancherythroculter nigrocauda(♂),“Xianfeng No.1”

给体质量22~88 g的杂交鲌“先锋1号”[翘嘴红鲌(♀)×黑尾近红鲌(♂)杂交子一代(F1)]幼鱼胸鳍基部注射6μg/g的植物血球凝集素,12~14 h后再注射8μg/g秋水仙素,2 h后尾静脉采血,经常规空气干燥、Giemsa染色、显微拍照及Photoshop CS 6软件分析等步骤研究“先锋1号”F1的染色体数目、核型等;用公鸡血细胞DNA绝对含量(2.50 pg/2C)作对照,用流式细胞仪测定“先锋1号”F1血细胞DNA含量,以丰富其细胞遗传学特征。试验结果显示,杂交鲌“先锋1号”的染色体数目为2n=48,核型公式为28m+18sm+2st,染色体臂数NF=94,未发现次缢痕和随体,也未发现与性别有关的异型性染色体;杂交鲌“先锋1号”的DNA含量为(3.16±0.04)pg/2C。杂交鲌“先锋1号”的染色体数目与父母本相同,核型与父本黑尾近红鲌更接近,DNA含量与父母本无显著性差异。

The chromosome number and karyotype were investigated in juvenile culter hybrid F1[Erythroculter ilishaeformis(♀)×Ancherythroculter nigrocauda(♂)](known as“Xianfeng No 1”)with body weight of 22—88 g injected with phytohemagglutinin(PHA)at a dose of 6μg/g,and injected with colchicine at a dose of 8μg/g after 12—14 h,and 2 h later,blood was sampled via caudal vein,routine air drying,Giemsa staining,photomicrography and Photoshop CS6 Software analysis and other steps.The cellular DNA content of culter hybrid F1,“Xianfeng No 1”,was determined by a flow cytometry via absolute content of DNA from rooster blood cells(2.50 pg/2C)as a control.The results showed that there were 48 chromosomes in culter hybrid F1,with karyotype formula of 28m+18sm+2st,and NF=94,without secondary constriction,satellite,heterosome and sex chromosome.The culter hybrid F1 had DNA content of(3.16±0.04)pg/2C,and the same chromosome number as their parents,with more similar karyotype to the male parent A.nigrocauda.There were no significant differences in the DNA content between the culter hybrid F1 and their parents.