十二烷基苯磺酸钠对黄河鲤急性毒性及血液生理生化指标的影响

Effects of Sodium Dodecylbenzene Sulfonate(LAS)on Acute Toxicity and Blood Biochemistry in Yellow River Carp Cyprinus carpio haematopterus

在水温(21.4±0.8)℃下,通过半静态生物毒性试验,在1.2 m×0.6 m×0.8 m水族箱中,研究表面活性剂十二烷基苯磺酸钠对体质量(38.7±0.8)g黄河鲤的96 h半致死质量浓度及血液生理生化指标的影响。试验结果显示,十二烷基苯磺酸钠对黄河鲤具有中等毒性,24、48、72、96 h的半致死质量浓度分别为10.27、9.51、9.16、8.59 mg/L。十二烷基苯磺酸钠质量浓度≥2.0 mg/L时,黄河鲤血浆总蛋白、白蛋白、球蛋白、血红蛋白质量浓度,红细胞、白细胞密度及血浆碱性磷酸激酶活性降低,血浆尿素氮和肌酐浓度,肌酸磷酸激酶、谷丙转氨酶和谷草转氨酶活性升高;十二烷基苯磺酸钠质量浓度≥3.0 mg/L时,黄河鲤血浆中Na^(+)、K^(+)浓度显著升高;十二烷基苯磺酸钠对血液Ca^(2+)浓度的影响较小。

The 96 h median lethal concentration(LC50)and blood physiological and biochemical indicators were investigated in Yellow River carp Cyprinus carpio haematopterus with body weight of(38.7±0.8)g exposed to surfactant sodium dodecylbenzene sulfonate(LAS)in a 1.2 m×0.6 m×0.8 m aquarium at water temperature of(21.4±0.8)℃through a semi-static biotoxicity test in order to explore the mechanism of physiological and biochemical toxicity of LAS on aquatic animals.It was found that LC50 of LAS to Yellow River carp was 10.27 mg/L in 24 h,9.51 mg/L in 48 h,9.16 mg/L in 72 h,and 8.59 mg/L in 96 h,with moderately toxicity of LAS to Yellow River carp.There were decrease in contents of plasma total protein(TP),albumin(ALB),globulin(GLB),red blood cell(RBC),white blood cell(WBC)and hemoglobin(Hb)and in plasma alkaline phosphate(ALP)activity at LAS concentration of≥2.0 mg/L,and increase in contents of plasma urea nitrogen(BUN)and creatinine(CREA),and activities of creatine phosphokinase(CPK),alanine aminotransferase(AST)and aspartate aminotransferase(ALT)in the Yellow River carp exposed to LAS.The elevated concentration of Na^(+)and K^(+)were observed in the plasma of Yellow River carp exposed to the LAS concentration of≥3.0 mg/L,the effect of LAS on plasma Ca^(2+)level being less.