静电纺CA/PpIX多孔纤维膜的制备及其光动力性能

Preparation and photodynamic performance of electrospinning CA/PpIX porous fibrous membrane

针对耐药性细菌感染问题,利用静电纺丝技术制备一种光动力广谱抗菌的醋酸/原卟啉(CA/PpIX)复合多孔超细纤维膜。利用扫描电子显微镜(SEM)观察纤维膜表面形貌,借助激光共聚焦扫描显微镜(CLSM)观察PpIX在醋酸纤维上的分布,采用傅里叶红外光谱仪(FT-IR)以及拉曼光谱仪(Raman)分析比较添加PpIX前后纤维膜基本化学结构的变化。通过DPBF底物氧化实验及材料对金黄色葡萄球菌和大肠杆菌的抗菌能力,探究材料的光动力性能,并且设计无机盐溶液(KI,NaNO_(2),MgCl_(2))梯度实验,以探究其与^(1)O_(2)的相互作用对抗菌性能的影响。结果表明,PpIX的加入并未改变纤维的基本形貌,但赋予了纤维膜良好的底物氧化及抗菌性能(光照30 min,对金黄色葡萄球菌的抗菌效果可达99.537%)。KI,MgCl_(2),NaNO_(2)溶液对CA/PpIX介导的光动力抗菌均具有一定的增强作用,其中KI溶液的增强作用最明显,其与^(1)O_(2)的相互作用产生了更多的自由基,显著增强了光动力抗菌效果,100 mmol/L的KI溶液可将纤维膜对两种菌的抗菌效率均提升至99.9999%。

To address the problem of drug-resistant bacterial infections,a photodynamic broad-spectrum antibacterial cellulose acetate/protoporphyrin(CA/PpIX)porous microfibre membrane was prepared by electrospinning.The surface morphology of the fibrous membrane was observed by scanning electron microscope(SEM)and the distribution of PpIX on CA membrane was observed by laser confocal scanning microscope(CLSM).The change of the basic chemical structure of membrane before and after loading PpIX was analyzed by Fourier infrared spectroscopy(FT-IR)and Raman spectrometer.Through the DPBF substrate oxidation experiment and the antibacterial ability against S.aureus and E.coli,the photodynamic performance of the material was explored,and the gradient experiment of inorganic salt solution(KI,NaNO_(2),MgCl_(2))was designed to explore the effect of its interaction with ^(1)O_(2) on the antibacterial performance.The results show that the loading of PpIX does not change the basic morphology of the fibrous membrane,but it gives the membrane good substrate oxidation and antibacterial properties(illumination 30 min,the antibacterial efficiency against S.aureus achieves 99.537%).KI,MgCl_(2)and NaNO_(2)solutions all have certain enhancement effects on photodynamic antibacterial mediated by CA/PpIX,among which KI solution has the most obvious enhancement effect.The interaction between the inorganic salt KI and ^(1)O_(2) produces more free radicals,which can significantly potentiate the photodynamic antibacterial effect.The 100 mmol/L KI solution can potentiate the antibacterial efficiency of the composite fibrous membrane against S.aureus and E.coli to 99.9999%.