妊娠期糖尿病产妇和胎儿胰岛素受体表达及DNA甲基化的改变

Expression of insulin receptor and changes in DNA methylation in women with GDM and fetus

目的探究妊娠期糖尿病(GDM)产妇和胎儿脂肪组织和血液中胰岛素受体(IR)表达和DNA甲基化的改变。方法选取2015年8月至2017年5月深圳市坪山区人民医院诊治60例实施剖宫产手术的产妇作为研究对象。其中30例GDM产妇纳入GDM组,30例正常糖耐量(NGT)产妇纳入对照组。通过PCR和蛋白质免疫印迹检测产妇腹腔内脏脂肪组织(VAT)和皮下脂肪组织(SAT)及空腹母体血(MB)中的IR基因和蛋白质表达;通过DNA甲基化分析检测母体(SAT、VAT、MB)和胎儿脐带血(CB)中IR启动子和内含子区的DNA甲基化水平。结果在SAT、VAT和血液中,GDM组IR mRNA/蛋白表达均降低(P<0.05)。DNA甲基化模式在组织间相似,并且在母亲和胎儿组间显示明显变化(P<0.05)。结论GDM产妇脂肪组织和血液中IR表达量降低,GDM产妇和胎儿DNA甲基化存在上升或下降的改变。

Objective To investigate the decreased expression of insulin receptor(IR)and changes in DNA methylation in adipose tissue and blood of women with gestational diabetes mellitus(GDM)and fetus.Methods 60 cases of cesarean section in Pingshan people′s Hospital of Shenzhen from August 2015 to May 2017 were selected as the research objects.Among them,30 GDM pregnant women were included in GDM group,and 30 normal glucose tolerance(NGT)pregnant women were included in control group.The expression of IR gene and protein in abdominal visceral adipose tissue(VAT),subcutaneous adipose tissue(SAT)and blood tissue samples were detected by PCR and Western blot.The DNA methylation levels of IR promoter and intron in maternal and fetal cord blood(CB)were detected by DNA methylation analysis.Results In SAT,VAT and blood,the expression of IR mRNA/protein decreased in GDM group(P<0.05).DNA methylation patterns were similar among tissues,and showed significant changes between maternal and fetal groups(P<0.05).Conclusions The expression of IR in adipose tissue and blood of GDM women decreased,and DNA methylation of GDM women and fetus increased or decreased.