摘要
目的建立HPLC法同时测定冠心丹参片(丹参、三七、降香)中丹参素钠、原儿茶醛、紫草酸、丹酚酸B、丹酚酸A、人参皂苷Rb1、二氢丹参酮、隐丹参酮、丹参酮Ⅰ、丹参酮Ⅱ_(A)的含量。方法该药物80%甲醇提取液的分析采用Waters SunFire C_(18)色谱柱(4.6 mm×150 mm,5μm);流动相乙腈-水(含0.2%磷酸),梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长203、240、270、280 nm。结果10种成分在各自范围内线性关系良好(r≥0.9995),平均加样回收率90.10%~105.87%,RSD 0.6%~2.8%。结论该方法稳定可靠,可用于冠心丹参片的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of danshensu sodium,protocatechualdehyde,lithospermic acid,salvianolic acid B,salvianolic acid A,ginsenoside Rb1,dihydrotanshinone,cryptotanshinone,tanshinoneⅠand tanshinoneⅡ_(A)in Guanxin Danshen Tablets(Salviae miltiorrhizae Radix et Rhizoma,Notoginseng Radix et Rhizoma,Dalbergiae odoriferae Lignum).METHODS The analysis of 80%methanol extract of this drug was performed on a 30℃thermostatic Waters SunFire C_(18) column(4.6 mm×150 mm,5μm),with the mobile phase comprising of acetonitrile-water(containing 0.2%phosphoric acid)flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 203,240,270,280 nm.RESULTS Ten constituents showed good linear relationships within their own ranges(r≥0.9995),whose average recoveries were 90.10%-105.87%with the RSDs of 0.6%-2.8%.CONCLUSION This stable and reliable method can be used for the quality control of Guanxin Danshen Tablets.
作者
王文晓
杨诺
高欢
任继国
乐世俊
WANG Wen-xiao;YANG Nuo;GAO Huan;REN Ji-guo;YUE Shi-jun(Key Laboratory for Compatibility of Traditional Chinese Medicine,Shaanxi Provincial Administration of Traditional Chinese Medicine,Shaanxi University of Chinese Medicine,Xi’an 712046,China)
出处
《中成药》
CAS
CSCD
北大核心
2021年第5期1141-1144,共4页
Chinese Traditional Patent Medicine
基金
国家自然科学基金青年基金项目(81903786)
陕西省自然科学基金项目(2019JQ-054)
陕西省高校科协青年人才托举计划项目(20190306)。
关键词
冠心丹参片
化学成分
HPLC
Guanxin Danshen Tablets
chemical constituents
HPLC