翘芩清肺剂中药免煎颗粒剂与饮片剂的抑菌作用比较

Comparative study on the bacteriostatic effect of Qiaoqin Qingfei Formula decocting-free granule and medicinal slices

目的观察翘芩清肺剂中药免煎颗粒剂及饮片煎煮剂的体外抑制金黄色葡萄球菌和鲍曼不动杆菌抑菌的效果,以评估其体外抑菌效果的差异,为翘芩清肺剂临床应用提供新的思路及科学依据。方法使用KB试纸扩散法和改良液体稀释法观察翘芩清肺剂中药免煎颗粒剂和饮片煎煮剂对金黄色葡萄球菌ATCC25923、鲍曼不动杆菌ATCC19606的最小抑菌浓度(MIC)。结果用KB法检测颗粒冲服剂和饮片煎煮剂金黄色葡萄球菌最低抑菌浓度为1.38 g/mL(P<0.01);鲍曼不动杆菌最低抑菌浓度为2.75 g/mL(P<0.01)。用改良液体稀释法冲服剂金黄色葡萄球菌最低抑菌浓度为10.0 g/mL(P<0.01);鲍曼不动杆菌最低抑菌浓度为5.5 g/mL(P<0.01)。煎煮剂两菌MIC均为5.5 g/mL(P<0.01)。饮片煎煮剂效果较冲服剂好(P<0.01)。结论翘芩清肺剂中药免煎颗粒冲服剂及饮片煎煮剂均可有效抑制金黄色葡萄球菌和鲍曼不动杆菌的生长,饮片煎煮剂的抑菌效果比免煎颗粒剂较优;对鲍曼不动杆菌的抑菌效果均较金黄色葡萄球菌弱,但无显著性差异性。

Objective To observe the antibacterial effects of Qiaoqinqingfei traditional Chinese medicine decoction and herbal concentrate-granules on Staphylococcus aureus(SA)and Acinetobacter baumannii(AB)in vitro.To assess the different antibacterial effects in vitro between them.It would provide a new idea and scientific basis for the clinical application of Qiaoqinqingfei Formula.Methods The KB test paper diffusion method and modified liquid dilution method were used to observe the minimum inhibitory concentration(MIC)of Qiaoqinqingfei traditional Chinese medicine decoction and herbal concentrate-granules on the Staphylococcus aureus ATCC25923 and Acinetobacter baumannii ATCC19606.Results The MIC of SA and AB in the two form of Qiaoqinqingfei were 1.38 g/mL(P<0.01)and 2.75 g/mL(P<0.01)by the KB test paper diffusion method.The MIC of SA and AB in herbal concentrate-granules of Qiaoqinqingfei were10.0 g/mL(P<0.01)and 5.5 g/mL(P<0.01)by the modified liquid dilution method.And the MIC of SA and AB in decoction both were 5.5 g/mL(P<0.01)in traditional Chinese medicine decoction of Qiaoqinqingfei.Decoction was better than granules(P<0.01).Conclusion Traditional Chinese medicine decoction and herbal concentrate-granules of Qiaoqinqingfei may both effectively inhibited the growth of SA and AB.The bacteriostatic effect of decoction was better than decoction-free granules.The bacteriostatic effect of AB was weaker than Staphylococcus aureus,but there was no significant difference between them.