蛹虫草对铜离子的耐受性和富集特征

Tolerance and Enrichment Characteristics of Cordyceps militaris to Cu^(2+)

为了解蛹虫草富集铜离子后对其自身生长和人体健康的影响,在培养基中添加不同浓度的铜离子,观察其对蛹虫草菌丝体及子实体生长、多糖含量以及铜离子富集量的影响。结果表明,铜离子对蛹虫草生长有一定抑制作用,最小铜离子添加量(1.6 mg/L)已经导致蛹虫草子实体出现矮小、分叉等退行性变化;蛹虫草的活性成分多糖含量随着铜离子添加量的增加而增加;铜离子添加量为3.2 mg/L时,菌丝体和子实体的铜离子富集率出现高峰值,分别为54.41%和191.01%。综上,最适宜的铜离子添加量为1.6~3.2 mg/L。在最小铜离子添加量1.6 mg/L下,每天食用29 g干蛹虫草或116 g鲜蛹虫草就能补足人体所需铜离子量。

To study the effects of Cu^(2+)enrichment on growth of Cordyceps militaris and human health,different concentration of Cu^(2+)was added into culture medium to observe the effects of Cu^(2+)on the growth of mycelia and fruiting bodies of C.militaris,the content of polysaccharides and Cu^(2+)enrichment.The results showed that Cu^(2+)inhibited the growth of C.militaris,because the addition of Cu^(2+)at the minimum concentration(1.6 mg/L)led to degenerative changes in the fruiting bodies of C.militaris,such as dwarfing and bifurcation.The polysaccharide content of C.militaris increased with the increase of Cu^(2+)addition.When the Cu^(2+)addition was 3.2 mg/L,the Cu^(2+)enrichment rate of mycelium and fruiting body was the highest,54.41%and 191.01%respectively.Therefore,by comparing the above factors,it is found that the optimum Cu^(2+)addition is 1.6—3.2 mg/L.With the minimum copper ion addition of 1.6 mg/L,29 g dry or 116 g fresh fruiting bodies are needed daily to supplement the amount of Cu^(2+)needed for people.