内脂素对老年大鼠脑组织损伤和炎症反应的影响分析

Analysis of the Effect of Visfatin on Brain Damage and Inflammationin Aged Rats

目的:探讨与分析内脂素对老年大鼠脑组织损伤和炎症反应的影响。方法:10个月龄老年健康雄性Sprague-Dawley(SD)大鼠60只随机平分为两组-对照组与内脂素组,内脂素组给予腹腔注射重组人内脂素200μg/次,对照组均给予等量蒸馏水灌胃,2次/w,连续给药4 w。对比两组给药前、给药第2 w、给药第4 w的逃避潜伏期、血清白介素(Interleukin,IL)-6与C-反应蛋白(C-reactive protein,CRP)含量,及给药第4 w的线粒体超氧化物歧化酶(Superoxide dismutase,SOD)与谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)活性、海马与皮层区神经元凋亡率。结果:所有大鼠在实验期间均出现典型的绕池壁现象。两组给药前逃避潜伏期、血清IL-6与CRP含量对比差异无统计学意义(P>0.05),给药后两组第2 w、给药第4 w的逃避潜伏期、血清IL-6与CRP含量均降低(P<0.05),且内脂素组给药第2 w、给药第4 w的上述指标低于对照组,对比均有统计学意义(P<0.05);内脂素组给药第4 w的线粒体SOD、GSH-Px活性高于对照组,海马与皮层区神经元凋亡率低于对照组,经对比差异有统计学意义(P<0.05)。结论:内脂素在老年大鼠的应用能缓解脑组织损伤,抑制炎症反应,有利于清除过量的自由基,降低神经元的凋亡率。

Objective: To explore and analyze the effect of visfatin on brain damage and inflammation in aged rats. Methods: 60 cases of 10-month-old healthy male Sprague-Dawley(SD) rats were equally randomly divided into two groups-the control group and the visfatin group. The visfatin group were given intraperitoneal injection of recombinant human visfatin 200 μg/time, the control group were given equal amount of distilled water by gavage, 2 times/week for 4 consecutive weeks. The escape latency, serum interleukin(IL)-6 and C-reactive protein(C-reactive protein, CRP) levels before administration, the 2 nd week of administration, and the 4 th week of administration were compared between the two groups. 4 weeks of mitochondrial superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activity, hippocampal and cortical neuronal apoptosis rate. Results: All rats were showed typical phenomenon around the pool wall during the experiment. There was no significant difference in the escape latency, serum IL-6 and CRP content between the two groups before administration(P>0.05). The escape latency, serum IL-6 and serum IL-6 and CRP levels in the 2 nd week and 4 th week after administration The content of CRP was reduced(P<0.05), and the above indicators of the visfatin group were lower than those of the control group on the second and fourth weeks of administration, and the comparison was statistically significant(P<0.05);the visfatin group Mitochondrial SOD and GSH-Px activities were higher than those in the control group on the 4 th week of administration, and the apoptosis rate of neurons in the hippocampus and cortex was lower than that in the control group. The difference was statistically significant(P<0.05). Conclusion: The application of visfatin in aged rats can alleviate brain tissue damage, inhibit inflammatory response, help to remove excess free radicals, and reduce neuronal apoptosis rate.