摘要
目的:建立高效液相色谱法同时测定白茶中6种茶多酚类物质含量的方法。方法:采用Agilent C18(250mm×4.6mm,5.0μm)色谱柱,流动相:A:0.3%甲酸水溶液;B:0.3%甲酸甲醇溶液;柱温:40℃;流速:1mL/min;检测波长:280nm;进样量:2μL;梯度程序:0min(85%A+15%B),60min(70%A+30%B)。结果:建立同时测定茶叶中6种茶多酚高效液相色谱定量分析方法,范围内线性关系良好。结论:利用响应面法优化茶叶中茶多酚提取工艺,采用高效液相色谱法同时测定不同茶叶中6种茶多酚活性成分,此方法可用来进行不同种类茶叶中茶多酚提取及定量分析研究。
Objective:In order to establish a method for simultaneous determination of six kinds of tea polyphenols in white tea by HPLC.Methods:parameters for using HPLC were:Agilent C18(250 mm×4.6 mm,5.0μm)column,Mobile phase:A:0.3%formic acid solution;B:0.3%formic acid methanol solution,the column temperature 28℃,the injection volume 2μL,flow rate 1.0 mL/min,and detection wavelength 280 nm.Results:The methods of simultaneous determination of 6 kinds of polyphenols in tea by HPLC was established.Conclusion:response surface methodology was used to optimize the extraction process of tea polyphenols in white tea.High performance liquid chromatography was used to determine the six active components of tea polyphenols in different tea.This method can be used to study the active components of tea polyphenols.
作者
于然
李晔
金丽华
陈亮
樊莹
刘蕊
李捷
YU Ran;LI Ye;JIN Li-hua;CHEN Liang;FAN Ying;LIU Rui;LI Jie(College of Bioengineering,Beijing Polytechnic,Beijing 100176)
出处
《中国食品添加剂》
CAS
北大核心
2021年第5期74-82,共9页
China Food Additives
基金
北京市教委科技一般项目(KM202010858002)
天然产物国家标准样品研制及产品开发技术研究(SG030801)。
关键词
茶多酚
高效液相色谱
响应面
定量测定
polyphenols
HPLC
response surface
quantitative determination
