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桑葚对非酒精性脂肪性肝病模型大鼠脂质代谢的影响 被引量:4

Effects of Mulberry on Regulation of Lipid Metabolism in a Rat Model of Non alcoholic Fatty Liver Disease
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摘要 目的观察桑葚对高脂饮食诱导的非酒精性脂肪性肝病(NAFLD)模型大鼠脂质代谢的影响。方法60只清洁级SD雄性大鼠按随机数字表法随机分为正常组10只(正常饲料)和造模组50只(高脂饲料),饲养8周。于第9周起,再将造模组大鼠按随机数字表法随机分为模型组、桑葚低(2.5g/kg)、中(5g/kg)、高(10g/kg)剂量组、异甘草酸镁对照组(0.015g/kg),每组10只。干预组灌胃给予对应药物,正常组与模型组给予等量生理盐水,持续6周。赖氏法测定大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、酶法测定血清三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)及高密度脂蛋白胆固醇(HDL-C)的变化,观察大鼠肝脏大体观,计算肝脏指数,油红O染色及HE染色观察肝组织脂滴分布状况及病理学变化。结果与正常组比较:模型组大鼠肝脏大体色泽较为苍白,肝脏指数下降[(2.86±0.10)比(3.79±0.55),P<0.01],肝脏油红O染色强阳性,脂肪沉积明显,肝脂肪变显著,血清ALT、AST、TG、TC及LDL-C升高,HDL-C下降[(72.50±6.20)U/L比(38.70±7.87)U/L、(267.00±40.70)U/L比(93.60±16.61)U/L、(1.52±0.24)mmol/L比(0.99±0.09)mmol/L、(1.52±0.14)mmol/L比(1.11±0.07)mmol/L、(0.57±0.04)mmol/L比(0.41±0.07)mmol/L、(0.38±0.05)mmol/L比(0.43±0.03)mmol/L,P<0.05]。与模型组比较:各干预组大鼠肝脏大体色泽的红润度均有一定改善,以桑葚高剂量组的改善最为明显;肝脏指数均有不同程度提高,桑葚中、高剂量组及异甘草酸镁对照组的改善显著[(3.12±0.14)、(3.22±0.10)及(3.16±0.22)比(2.86±0.10),P均<0.01]。肝组织油红O染色强度随桑葚剂量的增加而逐渐减轻,桑葚高剂量组及异甘草酸镁对照组的肝脏脂肪沉积改善显著;肝脂肪变均不同程度改善。桑葚各剂量组血清ALT、AST均下降,呈剂量依赖性[(54.80±5.16)U/L、(53.00±8.65)U/L、(43.30±2.36)U/L比(72.50±6.20)U/L,(135.40±26.68)U/L、(124.60±35.29)U/L、(93.90±8.91)U/L比(267.00±40.70)U/L,P均<0.01];TG、LDL-C均下降,呈剂量依赖性[(1.05±0.22)mmol/L、(0.95±0.17)mmol/L、(0.79±0.03)mmol/L比(1.52±0.24)mmol/L,(0.48±0.05)mmol/L、(0.47±0.05)mmol/L、(0.39±0.02)mmol/L比(0.57±0.04)mmol/L,P均<0.01];桑葚高剂量组血清TC降低[(1.21±0.08)mmol/L比(1.52±0.14)mmol/L,P<0.01],低、中剂量组HDL-C升高[(0.46±0.05)mmol/L及(0.43±0.04)mmol/L比(0.38±0.05)mmol/L,P<0.05或P<0.01]。结论桑葚对高脂饮食诱导NAFLD模型大鼠脂质代谢具有改善作用。 Objective To investigate the effects of mulberry on the regulation of lipid metabolism in a rat model of high-fat diet-induced non alcoholic fatty liver disease(NAFLD).Methods Sixty male Sprague-Dawley rats were randomly divided into negative control(n=10;fed with normal diet)and model groups(n=50;fed with high-fat diet)for 8 weeks.After that,the model group of rats was randomly assigned into model control,positive control(treated with magnesium isoglycyrrhizinate at 0.015g/kg dose)and intervention groups(low 2.5g/kg,moderate 5g/kg,and high doses 10g/kg of mulberry via oral gavage;all n=10)and treated for 6 weeks.The control mice were received the same amount of normal saline.After treatment,the levels of serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were measured using Reit′s method,and the levels of serum triglyceride(TG),total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),and high density lipoprotein cholesterol(HDL-C)were measured using enzymatic determination.The rat liver tissues were resected and subjected to the calculation of the liver indexes,the oil red O staining,and H&E staining for lipid droplets distribution and the pathologic changes.Results Compared with the negative control group,the liver of the model rats was pale and the liver index was decreased[(2.86±0.10)vs.(3.79±0.55);P<0.01];oil red O staining showed strong fat deposition and obvious liver steatosis;serum levels of ALT,AST,TC,TG,and LDL-C were all increased,whereas the HDL-C level was decreased[(72.50±6.20)vs.(38.70±7.87)U/L,(267.00±40.70)vs.(93.60±16.61)U/L,(1.52±0.24)vs.(0.99±0.09)mmol/L,(1.52±0.14)vs.(1.11±0.07)mmol/L,(0.57±0.04)vs.(0.41±0.07)mmol/L,(0.38±0.05)vs.(0.43±0.03)mmol/L,respectively;P<0.05].Furthermore,compared with the model group,liver morphology was improved in each intervention group,especially in the high dose mulberry group;the liver index was higher in each intervention group,and the improvements in the moderate and high dose mulberry treated rats and magnesium isoglycyrrhizate-treated rats were obvious[(3.12±0.14),(3.22±0.10),and(3.16±0.22)vs.(2.86±0.10);P<0.01].The intensity of the oil red O staining and liver steatosis was decreased in treatment groups in a dose-dependent fashion.Serum levels of ALT,AST,TG,and LDL-C were all decreased in the treatment groups in a dose-dependent manner[(54.80±5.16),(53.00±8.65),and(43.30±2.36)vs.(72.50±6.20)U/L;(135.40±26.68),(124.60±35.29),and(93.90±8.91)vs.(267.00±40.70),(1.05±0.22),(0.95±0.17),and(0.79±0.03)vs.(1.52±0.24)mmol/L;(0.48±0.05),(0.47±0.05),and(0.39±0.02)vs.(0.57±0.04)mmol/L,respectively;P<0.01],while serum TC level of high dose mulberry-treated group was decreased[(1.21±0.08)vs.(1.52±0.14)mmol/L;P<0.01],but serum HDL-C level in low and moderate dose mulberry-treated groups was increased[(0.46±0.05),and(0.43±0.04)vs.(0.38±0.05)mmol/L;P<0.05 and P<0.01,respectively]compared to the model control rats.Conclusion Mulberry extract showed a protective effect on the regulation of lipid metabolism in a rat model of high-fat diet-induced NAFLD.
作者 蒋焱 蒋素文 胡爱荣 孟依娜 陆佳林 何哲耘 JIANG Yan;JIANG Su-wen;HU Ai-rong;MENG Yi-na;LU Jia-lin;HE Zhe-yun(Ningbo Institute of Liver Diseases,Hwamei Hospital,University of Chinese Academy of Sciences,Ningbo 315010,China;Department of Liver Diseases,Shaoxing University-Affiliated Hospital,Shaoxing 312000,China;Department of Infectious Diseases,Ninghai First Hospital,The Medicare and Health Group,Ningbo 315600,China)
出处 《浙江中西医结合杂志》 2021年第5期407-412,共6页 Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金 浙江省中医药科技计划项目(No.2018ZB118) 浙江省宁波市消化系统肿瘤临床医学研究中心(No.2019A21003)。
关键词 大鼠 桑葚 非酒精性脂肪性肝病 脂质代谢 Rat Mulberry extract Nonalcoholic fatty liver diseases lipid metabolism
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