COTE-1上调Yap促进非小细胞肺癌细胞增殖和侵袭

COTE-1 accelerated NSCLC proliferation and invasion by up-regulating Yap

目的探讨COTE-1蛋白在非小细胞肺癌中的表达及对肺癌细胞增殖和侵袭的影响。方法免疫组织化学染色检测COTE-1在肺癌组织中的表达;克隆形成实验和Transwell实验检测COTE-1对肺癌细胞增殖和侵袭能力的影响;Western blot检测COTE-1对肺癌细胞YAP和CTGF蛋白表达的影响。结果 COTE-1在非小细胞肺癌组织和细胞系中明显高表达,COTE-1过表达促进肿瘤细胞增殖和侵袭能力,COTE-1过表达上调YAP及靶基因CTGF促进非小细胞肺癌的演进。结论 COTE-1参与调控YAP及靶基因CTGF的表达,可作为一个肺癌的潜在治疗靶点。

Objective To investigate the expression of COTE-1 in non-small cell lung cancer and its effect on proliferation and invasion of lung cancer cells. Methods The expression of COTE-1 in lung cancer tissue was detected by immunohistochemistry;the effect of COTE-1 on proliferation and invasion of lung cancer cells was detected by clone formation assay and Transwell assay;the expression of YAP and CTGF in lung cancer cells was detected by Western blot. Results COTE-1 was highly expressed in human non-small cell lung cancer samples and cell lines, whose overexpression promoted tumor cell proliferation and invasion. Ectopic expression of COTE-1 increased the expression of YAP as well as its downstream targets CTGF, CyclinD1 and CyclinE1, which in turn enhanced the proliferation and invasion of non-small cell lung cancer. Conclusion COTE-1 is involved in regulating the expression of YAP and CTGF, and might be a potential target for targeted therapy of cervical cancer.