艾康片减轻环磷酰胺心脏毒性研究

Study on Aikang Tablets Alleviating Cyclophosphamide-Induced Cardiotoxicity

目的:评价艾康片对环磷酰胺引起的心脏毒性的保护作用。方法:将40只BALB/c-nu小鼠随机分为正常组、模型组及艾康片高、中、低剂量组,每组8只。除正常组外,其余各组腹腔注射环磷酰胺0.3 g/kg制备环磷酰胺心脏毒性模型,每周1次,连续2周。模型制备当天,艾康片高、中、低剂量组按20 mL/(kg·d)的剂量分别灌胃浓度为0.046 g/mL、0.023 g/mL、0.012 g/mL的艾康片溶液,每天1次,连续给药2周;正常组、模型组在相同条件下灌胃等体积蒸馏水。检测各组小鼠血清乳酸脱氢酶(LDH)、羟丁酸脱氢酶(HBDH)、谷草转氨酶(AST)、肌酸激酶(CK)、肌酸激酶同工酶(CKMB)含量;计算心脏指数;HE染色观察心脏病理学改变。结果:与正常组比较,模型组小鼠心脏指数及血清LDH、HBDH均升高(P<0.05),心肌细胞肥大、心肌细胞变性、炎症细胞浸润Rank值及个体评分均增加(P<0.01)。与模型组比较,艾康片高、中、低剂量组心脏指数、血清LDH、HBDH均降低(P<0.01);艾康片高剂量组心肌细胞肥大Rank值及个体评分降低(P<0.05);艾康片中剂量组心肌细胞肥大、心肌细胞变性、炎症细胞浸润Rank值及个体评分均降低(P<0.05,P<0.01)。结论:艾康片对环磷酰胺引起的心脏毒性有保护作用。

Objective:To evaluate the protective effect of Aikang tablets on cyclophosphamide-induced cardiotoxicity.Methods:A total of 40 BALB/c-nu mice were randomly divided into the normal group,the model group,and the Aikang tablets groups of high-dose,medium-dose,and low-dose,8 mice in each group. Except for the normal group,in the other groups, cyclophosphamide-induced cardiotoxicity models were established by intraperitoneal injection of 0.3 g/kg cyclophosphamide for continuous two weeks,once a week. On the day of model establishment,the Aikang tablets groups of high-dose,medium-dose,and low-dose were given gastric perfusion of 0.046 g/mL,0.023 g/mL,and 0.012 g/mL solution of Aikang tablets respectively at the dose of 20 mL/(kg·d);the Aikang tablets groups received two weeks of continuous medication,once a day. The normal group and the model group were given gastric perfusion of distilled water of the same volume at the dose of 20 mL/(kg·d). The content of lactic dehydrogenase(LDH),hydroxybutyric dehydrogenase(HBDH), aspartate aminotransferase(AST), creatine kinase(CK), and creatine kinase isoenzyme(CKMB) in serum were detected in each group. Cardiac index was calculated. The change in cardiopathology was observed through HE staining.Results:Compared with those in the normal group,in the model group,LDH and HBDH in serum as well as cardiac index were increased(P<0.05);rank values of cardiomyocyte hypertrophy, cardiomyocyte degeneration, and inflammatory cell infiltration as well as individual score were increased(P<0.01). Compared with those in the model group,in the Aikang tablets groups of high-dose,medium-dose,and low-dose,LDH and HBDH in serum as well as cardiac index were decreased(P<0.01). Compared with those in the model group,rank value of cardiomyocyte hypertrophy and individual score in the highdose Aikang tablets group were decreased(P<0.05);rank values of cardiomyocyte hypertrophy, cardiomyocyte degeneration,and inflammatory cell infiltration as well as individual score were decreased in the medium-dose Aikang tablets group(P<0.05,P<0.01). Conclusion:Aikang tablets has the protective effect on cyclophosphamide-induced cardiotoxicity.