纤维素酶对柱花草青贮品质和营养成分的影响

Effect of cellulase on the quality and nutrient composition of Stylosanthes guianensis silage

为了探究纤维素酶对柱花草营养成分和青贮品质的影响,试验以热研2号柱花草为材料,设对照组(CK组)和4个纤维素酶添加组(S1~S4组),每组3个重复,对照组不添加纤维素酶,S1~S4组分别添加5,10,20,40 mg/kg纤维素酶,青贮30 d后取样分析营养成分和青贮品质。结果表明:S1~S4组的干物质含量和粗蛋白含量均显著高于对照组(P<0.05),中性洗涤纤维和酸性洗涤纤维含量均显著低于对照组(P<0.05)。与对照组相比,添加纤维素酶的S1~S4组能显著降低柱花草青贮饲料的pH值(P<0.05),S1~S4组的乳酸含量均显著高于对照组(P<0.05)。与对照组相比,S4组(添加40 mg/kg纤维素酶)的干物质、粗蛋白和乳酸含量分别提升了10%、12%和69%,中性洗涤纤维、酸性洗涤纤维含量及pH值降低了10%、8%和15%。说明添加纤维素酶可以有效改善柱花草青贮品质并提升营养价值,添加40 mg/kg纤维素酶较为适合。

In order to explore the effect of cellulase on the quality and nutritional composition of Stylosanthes guianensis silage, Stylosanthes guianensis cv. Reyan No.2 was divided into control(CK group) group and 4 cellulase addition groups(S1-S4 groups) with 3 repeats in each group. The control group did not add cellulase, and the S1-S4 groups was supplemented with 5, 10, 20 and 40 mg/kg cellulase. After 30 days of silage, the silage quality and nutritional composition were analyzed. The results showed that the dry matter content and crude protein content in S1-S4 groups were significantly higher than those of CK group(P<0.05), while the contents of neutral detergent fiber and acid detergent fiber were significantly lower than those in the CK group(P<0.05). The pH value of Stylosanthes guianensis silage in the S1-S4 groups were significantly lower than that in CK group(P<0.05), and the lactic acid content in the S1-S4 groups were significantly higher than that in the CK(P<0.05). Compared with the control group, the dry matter, crude protein and lactic acid content in the S4 group(adding 40 mg/kg cellulase) increased by 10%, 12%, and 69%, respectively, and the content of neutral detergent fiber, acid detergent fiber and pH decreased by 10%, 8% and 15%, respectively. The results indicated that the addition of cellulase to Stylosanthes guianensis could effectively improve the quality and nutritional value of silage, and the addition of 40 mg/kg cellulase was more suitable.