实验性延期再植牙大鼠模型组织学分析方法的建立

Establishment of Histological Analysis Method of Experimental Delayed Tooth Replantation Rat Model

目的:建立延期再植牙大鼠模型并观察牙根不同部位预后特点,比较不同组织学分析方法的合理性,为相关临床、基础研究提供参考。方法:6周龄雄性SD大鼠10只,右侧上颌切牙脱位后延期再植,术后30 d处死,分离上颌骨;利用micro CT从不同截面分析上颌切牙及牙周组织生理结构。切片后通过HE染色、MASSON染色、TRAP染色观察比较牙根不同部位愈合情况、破骨细胞分布情况。结果:大鼠切牙唇侧有釉质覆盖,腭侧牙骨质覆盖,且再植牙不同部位愈合情况、破骨细胞分布有差异,其牙根唇侧未见明显吸收,腭侧根中1/3相比根尖1/3和根颈1/3吸收范围更广、深度更深,破骨细胞分布数量更多,差异具有统计学意义(P<0.001),提出了腭侧中1/3作为牙根吸收组织学分析区域的合理性。结论:本实验比较了再植牙大鼠模型牙根不同部位的愈合情况,提出了一种较为合理的组织学分析方法,能客观反映再植牙愈合方式,为进一步研究再植牙愈合机理提供了较好的研究模型。

Objective:To establish a rat model of delayed tooth replantation and observe the prognostic characteristics of different root areas,compare the rationality of different histological analysis methods,and provide references for related clinical and basic research.Methods:Ten 6-week-old male Sprague-Dawley rats were replanted after the right maxillary incisor was extracted.They were sacrificed 30 days after the operation,and the maxilla was separated.The physiological structures of the maxillary incisors and periodontal tissue were analyzed from different sections by micro CT.After sectioning,HE staining,MASSON staining,and TRAP staining were used to observe the healing of different parts of the root and the distribution of osteoclasts.Results:The labial side of the rat incisor was covered by enamel,and the palatal side was covered by cementum.The healing situation and the distribution of osteoclasts were different in different parts of the replanted tooth.Among them,1/3 of the palatal side was suitable for the histology of root resorption.analysis.There was no obvious absorption on the labial side of the tooth root.Compared with the apical 1/3 and the cervical1/3,the absorption range and depth of the middle part was wider and deeper,and the number of osteoclasts was more distributed,in which the difference was statistically significant(P<0.001),suggesting the rationality that the middle 1/3 of the palatal side is suitable for histological analysis of root resorption.Conclusions:This experiment compares the healing of different parts of the tooth root in the replanted rat model,and proposes a more reasonable histological analysis method,which can objectively reflect the healing mode of the replanted tooth,and provides a better research model for further study about the healing mechanism of replanted teeth.