单唾液酸神经节苷酯对鱼藤酮诱导的大鼠中脑神经元细胞的保护作用

Protective Effect of Monosialoganglioside on Rat Midbrain Neurons Induced by Rotenone

目的研究单唾液酸神经节苷酯(GM1)对鱼藤酮诱导的帕金森(PD)模型大鼠的中脑神经细胞的保护作用。方法将由中国科学院上海实验动物中心提供的24只SD大鼠随机分成正常对照组、鱼藤酮组、GM1对照组及GM1治疗组,各6只。正常对照组不予以任何处理,鱼藤酮组于大鼠背部皮下注射鱼藤酮1.5 mg/kg,GM1治疗组于大鼠背部皮下注射鱼藤酮1.5 mg/kg,15 min后腹腔注射GM120 mg/kg,GM1对照组腹腔注射GM120 mg/kg,观察各组大鼠7 d内的行为学变化;以MTT法检测各组大鼠神经元细胞存活率,并以络氨酸羟化酶(TH)进行免疫荧光染色,观察各组神经元细胞的形态学变化。结果①正常对照组和GM1对照组大鼠无明显行为学无异常改变,鱼藤酮组大鼠出现行动缓慢、肢体颤动等异常行为学表现,GM1治疗组部分大鼠出现活动减少、摄食减少,但无明显竖尾、步态不稳,部分大鼠无明显行为学改变;②正常对照组、鱼藤酮组、GM1治疗组、GM1对照组细胞存活率分别为96.64%、48.51%、76.58%、94.82%,组间差异有统计学意义(P<0.05);③TH染色显示,对照组和GM1对照组神经元形态无损伤,神经元突触无损伤;鱼藤酮组阳性神经元数量减少,神经形态严重损伤,GM1治疗组阳性神经元的形状及突触数目、长度较鱼藤酮组改善。结论GM1能减轻鱼藤酮引起的神经元细胞的凋亡,维持细胞形态的完整性。

Objective To study the protective effect of monosialoganglioside(GM1)on the midbrain nerve cells of rotenone-induced Parkinson's(PD)model rats.Methods 24 SD rats provided by the Shanghai Experimental Animal Center of the Chinese Academy of Sciences were randomly divided into a normal control group,a rotenone group,a GM1 control group and a GM1 treatment group,with 6 each.The normal control group was not given any treatment.The rotenone group was injected with 1.5 mg/kg of rotenone subcutaneously on the back of the rat.The rats in the GM1 treatment group were injected with rotenone 1.5 mg/kg subcutaneously on the back of rats,and 15 min later,GM1 was injected intraperitoneally with 20 mg/kg,and the GM1 control group was injected intraperitoneally with GM120 mg/kg.Observing the behavioral changes of rats in each group within 7 d;The survival rate of rat neuronal cells in each group was detected by MTT method,and immunofluorescence staining was performed with tyrosine hydroxylase(TH)to observe the morphological changes of neuronal cells in each group.Results①The rats in the normal control group and the GM1 control group had no obvious behavioral changes.The rats in the rotenone group showed abnormal behavior such as slow movement and limb tremor.Some rats in the GM1 treatment group showed reduced activity and reduced food intake,but no obvious vertical tails and unsteady gait,and some rats had no obvious behavioral changes;②The cell survival rates of the normal control group,rotenone group,GM1 treatment group,and GM1 control group were 96.64%,48.51%,76.58%,and 94.82%,respectively,the difference between the groups was statistically significant(P<0.05);③TH staining showed that the morphology of neurons in the control group and the GM1 control group was not damaged,and the neuron synapses were not damaged;the number of positive neurons in the rotenone group was reduced,and the neuromorphology was severely damaged.The shape,number and length of synapses of the positive neurons in the GM1 treatment group were better than those in the rotenone group.Conclusion GM1 can reduce the apoptosis of neuronal cells caused by rotenone and maintain the integrity of cell morphology.