甜叶菊花和叶中酚类成分分析及含量测定

Analysis and Determination of Phenolic Compounds in Flowers and Leaves of Stevia rebaudiana Bertoni

以高效液相色谱-串联四极杆质谱分析仪,结合质谱分析及比对对照品或文献报道化合物的保留时间和质谱参数,在甜叶菊花和叶中鉴定了16种酚类化合物。建立一种利用高效液相色谱法测定甜叶菊中16种酚类化合物含量的方法,并对甜叶菊一新品种盛花期花和叶中酚类的含量进行测定。结果表明,甜叶菊花和叶中酚酸化合物以新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、异绿原酸C为主,异绿原酸A含量最高,绿原酸含量次之;花和叶中类黄酮化合物主要为芦丁、槲皮素-7-O-葡萄糖苷、木犀草素-7-O-葡萄糖苷、木犀草素-7-O-芸香糖苷、槲皮素-3-O-木糖苷、槲皮素-3-O-鼠李糖苷、芹菜素-7-O-葡萄糖苷、山奈酚-3-O-阿拉伯糖苷、山奈酚-3-O-鼠李糖苷,槲皮素-3-O-鼠李糖苷含量最高;盛花期甜叶菊花和叶中酚类总量达(22.31±0.51)mg/g和(91.11±2.32)mg/g。

A novel method that utilized high-performance liquid chromatography-tandem quadrupole mass spectrometric analysis identified a total of 16 phenolic compounds from the flowers and leaves of a new variety of Stevia rebaudiana Bertoni by comparing the retention times and mass spectra of samples with reference sub-stances and publications.The major phenolic acids were neochlorogenic acid,chlorogenic acid,cryptochloro-genic acid,isochlorogenic acid B,isochlorogenic acid A,and isochlorogenic acid C.A and isochlorogenic acid A with the higest content,followed by chlorogenic acid.The major flavonoids in the flower and leaf were rutin,quercetin-7-O-glucoside,luteolin-7-O-glucoside,luteolin-7-O-rutinoside,quercetin-3-O-xyloside,quercetin-3-O-rhamnoside,apigenin-7-O-glucoside,kaempferol-3-O-arabinoside,kaempferol-3-O-rhamnoside and quercetin-3-O-rhamnoside with the higest content in the flower and leaf.Total contents of phe-nolic compounds reached(22.31±0.51)mg/g in S.rebaudiana Bertoni flowers and(91.11±2.32)mg/g in leaves during the flowering phase.