摘要
该文从泡菜中筛选到一株高产亚硝酸盐还原酶的菌种N-6,经16SrDNA序列测序鉴定为鼠李糖乳杆菌(Lactobacillus rhamnosus)。通过优化培养基中不同碳源、氮源、磷源等营养成分、培养方式、培养温度等条件,鼠李糖乳杆菌N-6的最适培养基为:2%葡萄糖、0.5%酪蛋白胨、0.2%磷酸氢二钾,37℃通气培养,通过硫酸铵分级盐析、透析、二乙基氨基乙基纤维素(diethylaminoethylcellulose,DEAE)柱层析等方法对亚硝酸还原酶进行纯化,酶活力由60.83 U/mL提高到764.49 U/mL,有效地提高了酶纯度。
The strain N-6,which produces high levels of nitrite reductase,was screened from pickles.It was i-dentified as Lactobacillus rhamnosus by 16SrDNA sequencing.Nutrient composition(using different carbon,nitrogen,and phosphorus sources),cultivation method,cultivation temperature,and other conditions were op-timized.The optimal medium for L.rhamnosus N-6 was as follows:2%glucose,0.5%casein peptone,0.2%dipotassium hydrogen phosphate,aerobic culture at 37℃.Purification of nitrite reductase by ammonium sulfate gradient precipitations,dialysis,diethylaminoethylcellulose anion exchange chromatography,yielded an in-crease in enzyme activity from 60.83 U/mL to 764.49 U/mL,effectively improving the purity of nitrite reductase.
作者
付豪
邓艳芳
黄丽玲
赵丹
王昌涛
李萌
FU Hao;DENG Yan-fang;HUANG Li-ling;ZHAO Dan;WANG Chang-tao;LI Meng(Beijing Key Laboratory of Plant Resources Research and Development,College of Chemistry and Materials Engineering,Beijing Technology and Business University,Beijing 100048,China;Beijing Innovation Center for Food Nutrition and Human Health,Beijing Technology and Business University,Beijing 100048,China)
出处
《食品研究与开发》
CAS
北大核心
2021年第10期179-185,共7页
Food Research and Development
关键词
乳酸菌
亚硝酸盐
亚硝酸盐还原酶
培养条件优化
分离纯化
lactic acid bacteria
nitrite
nitrite reductase
optimization of culture condition
separation and pu-rification