与南方水稻黑条矮缩病毒外壳蛋白P10互作的寄主因子筛选

Screening of Host Factors and Interaction With Coat Protein P10 of Southern Rice Black Streak Dwarf Virus

为找到水稻中与南方水稻黑条矮缩病毒(SRBSDV)外壳蛋白P10(CP)相互作用的寄主蛋白,利用P10筛选水稻膜系统酵母双杂cDNA文库,寻找水稻中与P10互作的蛋白并结合生物信息学技术进行分析,回转验证。结果表明,筛选到10个可能与P10互作的水稻寄主蛋白,如胚发育晚期丰富蛋白Lea14-A、硫氧还原蛋白TRXh1、铁硫结合蛋白IscA以及真核生物起始翻译因子eIF-5A等。这些蛋白的发现说明水稻受到病毒侵染时,生长发育与营养物质的合成均受到影响。本研究结果为后续进一步研究P10蛋白的功能及其致病机制提供了理论依据。

In order to understand the biological function of P10 protein of southern rice black-streaked dwarf virus(SRBSDV),we screened rice cDNA yeast two-hybrid library by using P10 protein as a bait,and analyzed the results by the methods of bioinformatics techniques.Ten different clones that may interact with P10 was obtained,including late embryogenesis abundant protein Lea14-A,thioredoxin H1,iron-sulfur assembly protein IscA,eukaryotic translation initiation factor 5 A.Their interactions were further confirmed through one by one yeast two hybrid method.These results indicated that the growth and biosynthesis of nutrients in rice might be affected after virus infection.These results will provide a better understanding to of the rice virus pathogenicity.