植绒采样拭子联合显色培养基快速筛查医务人员鼻腔金黄色葡萄球菌定植的价值

The value of rapid screening of staphylococcus aureus nasal colonization of medical staff with flocked sampling swab combined with chromogenic medium

目的 探讨植绒采样拭子联合显色培养基快速筛查医务人员鼻腔金黄色葡萄球菌定植的价值.方法 使用Eswab多功能植绒采样拭子采集我院医务人员的鼻前庭标本150份,采用选择性显色琼脂培养基进行培养,通过质谱鉴定、耐药性分析及mecA基因检测对Sau定植快速筛查结果进行验证.结果 鼻腔Sau定植率为23.3%,鼻腔MRSA定植率为11.3%,Sau菌株的报阳时间为(16.5±3.2)h;鼻腔Sau定植快速筛查与质谱鉴定、耐药性分析及mecA基因检测的符合率分别为100.0%、100.0%、97.1%.结论 植绒采样拭子联合显色培养基是一种快速、精准的医务人员鼻腔Sau定植筛查方法.

Objective To discuss the value of rapid screening of staphylococcus aureus nasal colonization of medical staff with flocked sampling swab combined with chromogenic medium.Methods Eswab multifunctional flocked sampling swab was used to collect 150 nasal vestibular specimens from mediral staff in our hospital.The selective chromogenic agar medium was used for culture.The rapid screening results of Sau colonization were verified by mass spectrometry identification,drug resistance analysis and mecA gene test.Results The Sau nasal colonization rates were 23.3%(35/150),the MRSA nasal colonization rates were 11.3%(17/150),and the reporting time of Sau strain positive screening was(16.5±3.2)h.The coincidence rate of rapid screening of Sau nasal colonization was in conformity with mass spectrometry identification,drug resistance analysis and mecA gene test was 100.0%(35/35),100.0%(35/35),97.1%(34/35),respectively.Conclusion The flocked sampling swab combined with chromogenic medium was a rapid and accurate screening method for Sau nasal colonization of medical staff.