细菌RNA结合蛋白Hfq的DNA压缩和复制作用研究进展

Research progress on DNA compaction and replication of Hfq, a bacterial RNA binding protein

RNA结合蛋白(RNA-Binding Protein)Hfq是一种重要的细菌转录后调节因子,之前对Hfq的研究大多集中在该蛋白对小分子非编码RNA (Small Non-Coding RNA,sRNA)和mRNA的作用上。Hfq最典型的功能是促进sRNA与其靶标mRNA碱基配对,在转录后介导对RNA的稳定性和翻译的调控。此外,Hfq也能与多种蛋白质直接或间接相互作用。然而,近年来的研究表明,除了RNA和蛋白质,Hfq还可以与DNA相互作用,在DNA压缩(DNA Compaction)和DNA复制(DNA Replication)等多种DNA代谢过程中发挥直接或间接的调控作用。额外的靶标和功能的鉴定将进一步夯实Hfq作为细菌中多种代谢途径核心调控因子的重要地位,也表明该蛋白的功能并不局限于其在RNA和蛋白质代谢中的作用。本文总结了Hfq在DNA代谢调控中的近几年最新研究进展,并展望了其前景。

RNA-binding protein Hfq is an important post-transcription regulator of bacteria. Previous studies on Hfq mostly focused on the effects of this protein on small non-coding RNA(sRNA) and mRNA. The most typical function of Hfq is to promote the base pairing of sRNA with its target m RNA and mediate the regulation of RNA stability and translation after transcription. In addition, Hfq can interact directly or indirectly with a variety of proteins. However, recent studies have shown that in addition to RNA and proteins, Hfq can also interact with DNA and play a direct or indirect regulatory role in various DNA metabolism processes such as DNA compaction and DNA replication. Additional target and functional identification will further reinforce the importance of Hfq as a core regulator of multiple metabolic pathways in bacteria. It also indicates that the function of this protein is not limited to its role in RNA and protein metabolism. In this manuscript, the latest research progress of Hfq in the regulation of DNA metabolism in recent years is summarized and its prospect is prospected.