摘要
结合实践教学需求和生产实际问题,设计了从蛋清中提取溶菌酶的综合实验。以蛋清中溶菌酶为研究对象,采用离子交换树脂和盐析等方法提取溶菌酶,通过比较学生设计的多种方案的提取效果,探讨了树脂离子型式(钠型和氢型)、盐种类(硫酸铵和氯化钠)等因素对溶菌酶提取量和提取纯度的影响。该综合实验有助于培养学生严谨的科学研究态度和解决生产实际问题的能力,启发学生不盲从,不照搬,勤思考,有利于生物技术、生物工程和制药工程等专业的综合性和创新性人才培养。
In combination with needs of training talents and solving practical problems in biopharmaceutical production,a comprehensive experiment for preparation and determination of lysozyme derived from eggs was designed.Lysozyme from eggs was extracted and detected by ion-exchange resin and salting-out.Compared with those results from students’protocols,the effects of ionic type(Na+and H+)and salt solution(ammonium sulfate and sodium chloride)on concentration and purity of lysozyme were explored.This comprehensive experiment is helpful not only for cultivating undergraduates’rigorous scientific attitude to research and ability to resolve practical problems,but also contributing to train comprehensive and innovative talents.
作者
赵慧
高永峰
倪春林
周武艺
ZHAO Hui;GAO Yongfeng;NI Chunlin;ZHOU Wuyi(Biochemical Engineering and Pharmaceutical Experimental Teaching Demonstration Center,College of Materials and Energy,South China Agricultural University,Guangzhou 510642,China)
出处
《实验室研究与探索》
CAS
北大核心
2021年第4期189-192,196,共5页
Research and Exploration In Laboratory
基金
广东省本科高校教学质量与教学改革工程立项建设项目(粤教高函[2017]214号)
2019年广东省本科高校教学质量与教学改革建设项目(广东省教育厅2019.12.5一教学团队项目16号,特色专业项目10号)。
关键词
生物技术制药
溶菌酶提取
实验教学改革
创新人才培养
biotechnological pharmaceutics
preparation of lysozyme
reform of experiment teaching
cultivation of innovative talents
