电化学法检测细胞中的胸腺嘧啶和胞嘧啶

Detection of Thymine and Cytosine in Cellsby Electrochemical Method

用基于氧化石墨烯量子点/多壁碳纳米管/丝网印刷电极(GOQDs/MWCNTs/SPCE^(*))的电化学检测方法同时检测小鼠胚胎成纤维(BALB/c 3T3)细胞中的胸腺嘧啶和胞嘧啶,并考察富集电位、富集时间、 pH值对胸腺嘧啶和胞嘧啶标准品电化学行为的影响.实验结果表明:最佳检测条件为富集电位0,富集时间150 s,pH=7.4;电极对胸腺嘧啶和胞嘧啶的最低检测限分别为0.69,0.95μmol/L;该方法可同时灵敏检测BALB/c 3T3细胞中的胸腺嘧啶和胞嘧啶.

The electrochemical detection method based on graphene oxide quantum dots/multi walled carbon nanotubes/screen printed electrode(GOQDs/MWCNTs/SPCE*)was used to simultaneously detect thymine and cytosine in BALB/c 3T3 cells.The effects of enrichment potential,enrichment time and pH values on the electrochemical behavior of thymine and cytosine were investigated.The experimental results show that the optimal detection conditions are as follows:enrichment potential is 0,enrichment time is 150 s,pH=7.4.The detection limits of thymine and cytosine are 0.69,0.95μmol/L,respectively.The electrochemical detection method can be used to detect thymine and cytosine in BALB/c 3T3 cells simultaneously.