1株分离于青海省喜马拉雅旱獭的布鲁氏菌微量生化鉴定

Microbiochemical identification of a Brucella strain isolated from Marmota himalayana in Qinghai province,China

目的对2017年自青海省喜马拉雅旱獭组织中分离出的1株疑似布鲁氏菌菌株进行生化鉴定。方法采用细菌微量生化管和常规病原学鉴定,对分离出的菌株进行检测,同时与3种布鲁氏菌标准菌株的检测结果进行对比。结果微量生化鉴定显示,该菌株可氧化尿素,不氧化鸟氨酸、赖氨酸、D-半乳糖、山梨醇、木糖、葡萄糖酸盐,为羊种布鲁氏菌。结论细菌微量生化鉴定方法快速简单,可为布鲁氏菌检验提供一个快速的辅助实验依据。

Objective To perform microbiochemical identification of a suspected Brucella strain isolated from Marmota himalayana in Qinghai province,China in 2017.Methods The isolated bacteria strains were detected by microbiochemical tube and routine pathogenic identification,and the results were compared with the results of three standard Brucella strains.Results Microbiochemical identification showed that the strain could oxidize urea and did not oxidize ornithine,lysine,D-galactose,sorbitol,xylose,and gluconate.Therefore,it was identified as Brucella melitensis.Conclusion Microbiochemical identification of bacteria is rapid and simple,and can provide an experimental basis for the detection of Brucella.