张家口市汉族围孕期女性细胞色素P450和谷胱甘肽硫转移酶基因多态性分析

Polymorphism analysis of cytochrome P450 and glutathione sulfur transferase gene in Han nationality perinatal women in Zhangjiakou

目的了解河北省张家口市汉族围孕期女性细胞色素P450 1A1基因(CYP1A1)和谷胱甘肽硫转移酶基因M1(GSTM1)、T1(GSTT1)和P1(GSTT1)多态性分布特征。方法选取2018年8月-2020年10月在张家口市妇幼保健院进行围孕期保健的327名汉族健康女性为研究对象。采集口腔黏膜上皮脱落细胞,提取基因组DNA。使用荧光定量PCR方法检测CYP1A1、GSTM1、GSTT1和GSTP1基因多态性,并进行统计分析。结果 CYP1A1 Ile462Val基因位点野生纯合子AA基因型频率为64.2%,突变杂合子AG基因型频率为30.9%,突变纯合子GG基因型频率为4.9%,A等位基因频率为79.7%,G等位基因频率为20.3%;GSTP1 A313G基因位点野生纯合子AA基因型频率为59.3%,突变杂合子AG基因型频率为35.5%,突变纯合子GG基因型频率为5.2%,A等位基因频率为77.1%,G等位基因频率为22.9%;GSTM1缺失基因型频率为46.5%,非缺失基因型频率为53.5%,GSTT1缺失基因型频率为41.6%,非缺失基因型频率为58.4%。结论获取张家口市汉族围孕期女性细胞色素P450基因和谷胱甘肽硫转移酶系相关基因多态性的群体遗传学特征,其中GSTM1和GSTT1相关基因位点缺失人群占比较高,此类人群对环境毒物的解毒能力低下,更应主动规避不良环境暴露,并采用个体化营养干预来抵抗环境毒物带来的不良后果。

Objective To understand the distribution characteristics of cytochrome P450 1A1 gene(CYP1A1) and glutathione sulfur transferase gene M1(GSTM1),T1(GSTT1) and P1(GSTT1) polymorphisms in peri-pregnancy females of Han nationality in Zhangjiakou,Hebei Province.Methods From August 2018 to October 2020,327 healthy women of Han nationality who underwent peri-pregnancy care in Zhangjiakou Maternal and Child Health Hospital were selected as the research subjects.Collect exfoliated cells of oral mucosa epithelium and extract genomic DNA.Fluorescence quantitative PCR method was used to detect CYP1A1,GSTM1,GSTT1 and GSTP1 gene polymorphism,and statistical analysis was performed.Results The wild homozygous AA genotype frequency of CYP1A1 Ile462 Val gene locus was 64.2%,the mutant heterozygous AG genotype frequency was 30.9%,the mutant homozygous GG genotype frequency was 4.9%,the A allele frequency was 79.7%,G allele frequency is 20.3%;wild homozygous AA genotype frequency of GSTP1 A313 G gene locus is59.3%,mutant heterozygous AG genotype frequency is 35.5%,mutant homozygous GG genotype frequency is 5.2%,A allele frequency The frequency of G allele was 77.1%,the frequency of G allele was 22.9%;the frequency of GSTM1 deletion genotype was 46.5%,the frequency of non-deletion genotype was 53.5%,the frequency of GSTT1 deletion genotype was 41.6%,and the frequency of non-deletion genotype was 58.4%.Conclusion The population genetic characteristics of the cytochrome P450 gene and glutathione sulfur transferase-related gene polymorphisms of Han nationality in Zhangjiakou during pregnancy were obtained.Among them,the population with missing GSTM1 and GSTT1 related gene loci accounted for a relatively high proportion.The detoxification ability of environmental toxins is low,and it is necessary to actively avoid adverse environmental exposures and adopt individualized nutritional interventions to resist the adverse consequences of environmental toxins.