基因芯片技术在耐药肺结核临床诊断中的应用

Application of Gene Chip Technology in Clinical Diagnosis of Drugresistant Pulmonary Tuberculosis

目的研究基因芯片技术应用于耐药肺结核早期诊断及耐性识别的临床意义。方法随机选取2018年12月-2019年12月本院收治的98例培养标本及抗酸染色标本呈阳性患者作为研究对象,采用基因芯片技术进行分支杆菌鉴别、利福平及异烟肼耐药性检测。结果98例样本中检测出结核分支杆菌复合群(mycobacterium tuberculosis,MTB)74株,非结核分支杆菌(nontuberculosis mycobacteria,NTM)15株,总检出率90.82%;耐药检测方面,单耐利福平5株,单耐异烟肼3株,耐多药10株;利福平最高突变率发生在531位点(46.67%),异烟肼最高突变率发生在315位点(84.62%)。结论基因芯片技术可以对结核分支杆菌进行快速、准确鉴别,并应用于利福平及异烟肼的耐药检测,为临床诊断结核病及非结核分支杆菌感染提供可靠依据。

Objective To study the clinical significance of gene chip technology in the early diagnosis and tolerance recognition of drugresistant tuberculosis.Methods 98 culture samples and positive acid fast staining samples from December 2018 to December 2019 in our hospital were randomly selected as the research objects.The identification of mycobacterium,rifampicin and isoniazid resistance were detected by gene chip technology.Results 74 strains of mycobacterium tuberculosis(MTB)and non mycobacterium tuberculosis were detected in 98 samples,15 strains were NTM,the total detection rate was 90.82%;5 strains were rifampicin resistant,3 isoniazid resistant and 10 multi drug resistant;the highest mutation rate of rifampicin was at site 531(46.67%),and the highest mutation rate of isoniazid was at site 315(84.62%).Conclusion Microarray technology can be used to identify mycobacterium tuberculosis rapidly and accurately,and to detect the drug resistance of rifampin and isoniazid,so as to provide a reliable basis for clinical diagnosis of tuberculosis and non mycobacterium tuberculosis infection.