双重实时荧光定量PCR法检测腹泻中病毒的价值

The Value of Double Real-time Fluorescence Quantitative PCR in Detecting Virus in Diarrhea

目的研究腹泻患者粪便中诺如病毒(GⅠ+GⅡ)行双重实时荧光定量聚合酶链式反应(polymerase chain reaction,PCR)法检测的价值。方法研究2019年1月-2020年3月我市人民医院送检的腹泻患者的粪便标本共400份,均接受双重实时荧光定量PCR法检测,GⅠ型诺如病毒特异性采用Taqman探针的5’端标记为FAM,GⅡ型诺如病毒特异性采用Taqman探针的5’端标记为VIC,立于同一反应管中,同时检测、分型诺如病毒,分析最终的病毒检出率。结果GⅠ型诺如病毒感染有15例,检出率是3.75%;GⅡ型诺如病毒感染有59例,检出率是14.75%;GⅠ型、GⅡ型诺如病毒混合感染有6例,检出率是1.50%,诺如病毒的总检出率是20.00%。结论腹泻患者的粪便采用双重实时荧光定量PCR法检测具有较高的临床应用价值。

Objective To study the value of dual real-time fluorescence quantitative polymerase chain reaction(PCR)detection of Norovirus(GⅠ+GⅡ)in stool of diarrhea patients.Methods A total of 400 fecal samples of diarrhea patients from January 2019 to March 2020 were studied,they were all tested by dual real-time fluorescence quantitative PCR method.The GⅠtype Norovirus was specifically labeled with the 5'end of Taqman probe as FAM,GⅡtype Norovirus specificity is labeled as VIC at the 5'end of the Taqman probe,which facilitates the simultaneous detection and typing of Norovirus in the same reaction tube,and analyzes the final virus detection rate.Results There were 15 cases of GⅠtype Norovirus infection,the detection rate was 3.75%;59 cases of GⅡtype Norovirus infection,the detection rate was 14.75%;GⅠtype,GⅡtype Norovirus mixed infection had 6 cases,the detection rate is 1.50%,and the total detection rate of Norovirus is 20.00%.Conclusion The detection of feces in patients with diarrhea by double real-time fluorescence quantitative PCR method has high clinical application value.