扶正抗癌汤免疫调节及抑制大鼠卵巢癌移植瘤生长的作用

Effect of Fuzheng Kangai Decoction on Immune Regulation and Inhibition of Tumor Growth in Rats with Ovarian Carcinoma

目的:探讨扶正抗癌汤(FZKAD)的免疫调节及抑制大鼠卵巢癌移植瘤生长的作用。方法:昆明种小鼠随机分为正常组,香菇多糖(0.05 g·kg^(-1))组及扶正抗癌汤高、中、低剂量(27.3,13.65,6.825 g·kg^(-1))组,每组10只;测定血清半数溶血值(HC50),抗体生成细胞水平及单核巨噬细胞吞噬功能等指标。Fischer 344大鼠右腋窝皮下接种卵巢癌NUTU-19细胞悬液,建立移植瘤模型,并随机分为模型组,顺铂组(0.002 g·kg^(-1))及扶正抗癌汤高、中、低剂量(18.9,9.45,4.725 g·kg^(-1))组,每组10只;另随机选择10只健康大鼠作为正常组。给药14 d后检测瘤质量,抑瘤率,T淋巴细胞亚群,血清细胞因子表达及肿瘤组织X盒结合蛋白1(XBP1),增强子结合蛋白同源蛋白(CHOP)表达。结果:与正常组比较,扶正抗癌汤高、中、低剂量组小鼠HC50,抗体生成细胞水平,吞噬指数和吞噬活性均明显增加(P<0.05,P<0.01)。与模型组比较,扶正抗癌汤高、中、低剂量组大鼠瘤质量及XBP1蛋白表达显著降低(P<0.01),而抑瘤率,CD4^(+),CD8^(+)T细胞比例,CD4^(+)/CD8^(+),肿瘤坏死因子-α(TNF-α),白细胞介素-2(IL-2),γ-干扰素(IFN-γ)表达及CHOP蛋白表达均明显增加(P<0.05,P<0.01)。结论:扶正抗癌汤具有提高正常小鼠免疫功能的作用,同时,也具有较好抑制大鼠卵巢癌移植瘤生长作用;免疫调节作用是扶正抗癌汤发挥抗卵巢癌作用的主要机制。

Objective: To explore the effect of Fuzheng Kangai decotion(FZKAD)on the immune regulation and the inhibition of tumor growth in rats with ovarian carcinoma. Method: Kunming mice were randomly divided into normal group, lentinan(0.05 g·kg^(-1)) group, and high(27.3 g·kg^(-1)), medium(13.65 g·kg^(-1))and low(6.825 g·kg^(-1))dose groups of FZKAD,with 10 mice in each group,serum hemolytic value(HC50),antibody-forming cells and the phagocytosis of mononuclear macrophages were measured. Fischer344 rat xenograft model was established through inoculation of NUTU-19 cell in the right axilla,and the model rats were randomly divided into model group,cisplatin group(0.002 g·kg^(-1)),and high(18.9 g·kg^(-1)),medium(9.45 g·kg^(-1)),and low(4.725 g·kg^(-1))dose groups of FZKAD,with 10 rats in each group,in addition,10 healthy rats were randomly selected as the normal group. Tumor quality,tumor inhibition rate,T lymphocyte subsets,and expressions of serum cytokines,enhancer binding protein homologous protein 1(XBP1)and enhancer binding protein homologous protein(CHOP)protein in tumor tissues were detected after 14 days of administration. Result: Compared with normal group,HC50,level of antibody-forming cells,phagocytic index and phagocytic activity of mice in high,medium and low-dose groups of FZKAD were significantly increased(P<0.05,P<0.01). Tumor quality and XBP1 protein expression in high,medium and low-dose groups of FZKAD were significantly decreased(P<0.01)compared with the model group,while the tumor inhibition rate,CD4^(+),CD8^(+)T cell ratio, CD4^(+)/CD8^(+)ratio, tumor necrosis factor-α(TNF-α), interleukin-2(IL-2),γ-interferon(IFN-γ)expression and CHOP protein expression were significantly increased(P<0.05,P<0.01).Conclusion: FZKAD can improve the immune function of normal mice and inhibit the tumor growth in rats with ovarian carcinoma,and the immunity regulation effect is the main mechanism.