损伤相关分子模式分子指标与年龄和体重指数的相关性研究

Correlation of damage-associated molecular pattern molecules with age and body mass index

目的探讨损伤相关分子模式(DAMPs)分子血清S100、C反应蛋白(CRP)、血清淀粉样蛋白A(SAA)及尿酸(UA)水平与年龄及体重指数(BMI)的相关性。方法采用观察性研究方法,选择2020年5至10月湖南省第二人民医院体检中心的体检人群作为研究对象,依照纳入标准入选健康体检者366名(男性131名,女性235名),按照年龄间隔20岁分为3个年龄段组,其中20~40岁156名(男性53名,女性103名),41~60岁110名(男性36名,女性74名),61~80岁100名(男性42名,女性58名)。采用Kruskal-Wallis H检验比较不同年龄段组之间血清S100、CRP、SAA及UA水平的差异。根据《中华人民共和国卫生行业标准-成人体重判定》,以BMI=24 kg/m^(2)为界,将研究对象分为非超重组(BMI<24 kg/m^(2))和超重组(BMI≥24 kg/m^(2))两组,采用1∶1倾向性评分匹配年龄、性别,非超重组96名[男性43名,女性53名,年龄52(35,66)岁],超重组96名[男性44名,女性52名,年龄52(36,64)岁],采用Mann-Whitney U检验比较两组之间血清S100、CRP、SAA及UA水平的差异。结果366名健康体检者中男、女血清UA水平中位数分别为356和277μmol/L,男性高于女性(Z=-10.428,P<0.001);男、女血清SAA水平中位数分别为3.1 mg/L和4.4 mg/L,女性高于男性(Z=3.652,P<0.001)。3个年龄段组血清S100中位数分别为0.058、0.057及0.070μg/L,血清CRP中位数分别为0.32、0.58及0.93 mg/L,血清SAA中位数分别为3.2、4.0及5.2 mg/L,血清UA分别为(301.8±61.5)、(298.6±69.8)及(329.0±77.8)μmol/L。61~80岁年龄段组血清S100、CRP、SAA、UA均显著高于20~40岁年龄组(H=-2.749,H=-6.731,H=-5.033,H=-2.521,P=0.018,P<0.001,P<0.001,P=0.035)和41~60岁年龄组(H=-2.719,H=-2.539,H=-2.540,H=-2.486,P=0.020,P=0.033,P=0.033,P=0.039);41~60岁组血清CRP水平显著高于20~40岁组(H=-4.108,P<0.001),20~40岁组与41~60岁组血清S100、SAA及UA水平比较差异无统计学意义(H=0.189,H=-2.360,H=-0.165,P=1.000,P=0.055,P=1.000);血清CRP、SAA水平与年龄呈正相关(r_(s)=0.342,r_(s)=0.301,P<0.001,P<0.001)。超重组、非超重组血清S100中位数分别为0.065和0.059μg/L,血清CRP中位数分别为0.92和0.47 mg/L,血清SAA中位数分别为5.0 mg/L和4.1 mg/L,血清UA中位数分别为339.5和301.5μmol/L;超重组血清CRP、SAA及UA水平均显著高于非超重组(Z=4.278,Z=2.025,Z=3.787,P<0.001,P=0.043,P<0.001),超重组血清S100高于非超重组但差异无统计学意义(Z=0.862,P=0.388),血清CRP、UA水平与BMI呈正相关(r_(s)=0.348,r_(s)=0.264,P<0.001,P=0.009)。结论随着年龄的增长,健康者血清S100、CRP、SAA及UA可能呈上升趋势,尤其血清CRP和SAA水平与年龄呈正相关;超重者血清S100、CRP、SAA及UA水平可能高于非超重者,尤其血清CRP、UA水平与BMI呈正相关。

Objective To explore the correlation of damage-associated molecular pattern molecules(DAMPs)serum S100,C-reactive protein(CRP),serum amyloid A(SAA)and uric acid(UA)with age and body mass index(BMI)to provide direction for further study of metabolic inflammation and inflammaging.Methods The observational study method was used,and three hundred and sixty-six healthy people(131 males and 235 females)were selected from the physical examination center of the Second People′s Hospital of Hunan Province from May to October 2020.They were divided into three age groups according to the age interval of 20 years,including 156(53 males and 103 females)aged 20-40 years,110(36 males and 74 females)aged 41-60 years,and 100(42 males and 58 females)aged 61-80 years.Kruskal Wallis H test was used to compare the differences of serum S100,CRP,SAA and UA levels among different age groups.According to the Health Industry Standards of the People′s Republic of China-Weight Determination for Adults,the boundary is BMI=24 kg/m^(2).The healthy people were divided into non overweight(BMI<24 kg/m^(2))and overweight(BMI≥24 kg/m^(2))two groups.The 1∶1 propensity score was used to match the age and gender.There were 96 non overweight subjects[43 males,53 females,age 52(35,66)years],96 overweight subjects[44 males,52 females,age 52(36,64)years].The serum levels of S100,CRP,SAA and UA in different BMI groups were compared by Mann-Whitney U test.Results The median serum UA concentrations in males and females were 356 and 277μmol/L,and the levels of serum UA of male was significantly higher than that of female(Z=-10.428,P<0.001);the median serum SAA concentrations in males and females were 3.1 mg/L and 4.4 mg/L,while the serum SAA level of female was significantly higher than that of male(Z=3.652,P<0.001);for 20-40,41-60,and 61-80 years old group,the median concentration of serum S100 was 0.058,0.057,0.070μg/L,and the median concentration of serum CRP was 0.32,0.58,0.93 mg/L;the median serum SAA concentrations were 3.2,4.0,5.2 mg/L;serum uric acid concentrations were(301.8±61.5),(298.6±69.8),(329.0±77.8)μmol/L.The levels of serum S100,CRP,SAA,UA in 61-80 years group were significantly higher than those of 20-40 years group(H=-2.749,H=-6.731,H=-5.033,H=-2.521,P=0.018,P<0.001,P<0.001,P=0.035)and 41-60 years old group(H=-2.719,H=-2.539,H=-2.540,H=-2.486,P=0.020,P=0.033,P=0.033,P=0.039).The levels of serum CRP of 41-60 years group was significantly higher than that of 20-40 years group(H=-4.108,P<0.001).There was no significant difference in levels of serum S100,SAA and UA between 20-40 years group and 41-60 years group(H=0.189,H=-2.360,H=-0.165,P=1.000,P=0.055,P=1.000);the levels of serum CRP and SAA were positively correlated with age(r_(s)=0.342,r_(s)=0.301,P<0.001,P<0.001);for overweight,non-overweight group,the median concentrations of serum S100 were 0.065μg/L,0.059μg/L,the median concentrations of serum CRP were 0.92 mg/L,0.47 mg/L,the median concentrations of serum SAA were 5.0 mg/L,4.1 mg/L,the median concentrations of serum UA were 339.5μmol/L,301.5μmol/L,the levels of CRP,SAA and UA in the overweight group were higher than those in the non-overweight group(Z=4.278,Z=2.025,Z=3.787,P<0.001,P=0.043,P<0.001);the levels of S100 in the overweight group was higher than those in the non-overweight group,but there was no significant difference in S100 between the two groups(Z=0.862,P=0.388);the levels of Serum CRP and UA were positively correlated with BMI(r_(s)=0.348,r_(s)=0.264,P<0.001,P=0.009).Conclusions With the increase of age,the serum S100,CRP,SAA and UA levels of healthy people may be on the rise,especially the serum CRP and SAA levels are positively correlated with age;the serum S100,CRP,SAA and UA levels of overweight people may be higher than those of non-overweight people,especially the serum CRP,UA levels are positively correlated with BMI.