蛛网膜下腔出血大鼠鼻咽全氟化合物处理对其神经保护作用研究

Neuroprotective Effect and Mechanism of NP-PFC Treatment in Rats with Subarachnoid Hemorrhage

[目的]探究蛛网膜下腔出血大鼠鼻咽全氟化合物处理(NP-PFC)对其神经保护作用及作用机制。[方法]40只大鼠经造模后随机均分为模型组(B组)、NP-PFC处理组(C组),另随机选取20只大鼠作为假手术组(A组)。通过对各组大鼠进行神经功能评价.脑组织切片HE染色、MDA含量的测定、SOD及CAT活性检测、脑组织RT-PCR实验、脑组织Wenstern blot实验,对NP-PFC的神经保护作用进行探究。[结果]A、B.C组大鼠的神经功能评分分别为(16.9±2.4)分.(11.6±2.2)分.(15.1±2.4)分,B组.C组大鼠神经功能评分低于A组,C组高于B组,差异具有统计学意义(F=26.64,P<0.001)。A组大鼠前额叶皮质中各层细胞结构清晰,神经细胞边缘清晰;B组大鼠前额叶皮质中各层细胞排列稀疏散乱,神经细胞边缘模糊;C组大鼠前额叶皮质中各层细胞排列情况较B组大鼠相比明显改善,其神经细胞边缘也较B组大鼠清晰。三组大鼠脑组织中MDA.SOD及CAT含量比较,差异具有统计学意义(F=331.94.473.27.275.82,P<0.05)。A.B,C三组大鼠脑组织中caspase-3 mRNA表达量分别为(0.63±0.11).(1.42±0.26).(0.77±0.19),差异具有统计学意义(F=46.04.P<0.001)。A.B.C三组大鼠脑组织中caspase-3蛋白表达量分别为(0.32±0.08).(0.72±0.13)、(0.39±0.09),差异具有统计学意义(F=43.60,P<0.001)。[结论]NP-PFC可通过抑制氧化应激及神经细胞的凋亡保护蛛网.膜下腔出血大鼠的神经系统。

[Objective]To explore the neuroprotective effect and mechanism of nasopharynx perfluorochemical(NP-PFC)treatment in rats with subarachnoid hemorrhage(SAH).[Methods]After modeling,forty rats were randomly divided into two groups:model group(group B),NP-PFC treatment group(group C),and another 20 rats were randomly selected as sham operation group(group A).After administration,we evaluated neurological function and morphology of prefrontal cortex(HE staining).The number of apoptotic cells was measured by TUNEL staining.Then.we detected the content of MDA and the activity of SOD and CAT.Finally,the expres-sion of caspase-3 mRNA and caspase-3 protein in brain tissue were detected by PCR and Western Blotting.[Re-sults]The neurological function scores of rats in groups A.B,and C were(16.9+2.4),(11.6±2.2),and(15.1±2.4)points,respectively.The neurological function scores of rats in groups B and C were lower than those in group A,while the score in the group C was higher than that in the group B;and the difference was statistically significant(F=26.64,P<0.001).The cell structure of each layer in the prefrontal cortex of the group A rats was in normal pattern,and the edges of nerve cells are clear.The cells of each layer in the prefrontal cortex of group B rats were sparsely arranged,and the edges of nerve cells were fuzzy.When NP PFC was used,the messy arrangement in the group C was significantly improved compared to the group B,and the edges of nerve cells were clearer than that of group B.Comparison of the contents of MDA.SOD and CAT in the brain tissues of the three groups of rats showed statistically significant differences(F=331.94,473.27.275.82,P<0.05).The expres-sion of caspase-3 mRNA in the brain tissues of rats in groups A,B and C were(0.63±0.11),(1.42±0.26),(0.77±0.19),respectively.and the difference was statistically significant(F=46.04.P<0.001).The expression of caspase-3 protein in the brain tissues of rats in groups A.B and C were(0.32±0.08),(0.72±0.13),(0.39±0.09),respectively,and the difference was statistically significant(F=43.60,P<0.001).[Conclusion]NP-PFC has neuroprotective efect on the morphology of brain tissue in SAH rats.And it can decrease the quantity of apop-totic cells through oxidative stress inhibition.