丝素蛋白/金纳米棒复合水凝胶的构建及其光热性能

Construction and photothermal properties of composite hydrogel based on silk fibroin and gold nanorod

针对皮肤表面肿瘤手术切除时残留癌细胞导致肿瘤复发的医学难题,以天然高分子丝素蛋白为基体材料,包覆光热试剂金纳米棒(AuNR),制备复合水凝胶,对术后残余肿瘤癌细胞进行光热消融,以防止肿瘤复发。采用辣根过氧化物酶(HRP)对丝素蛋白分子链进行交联制备丝素蛋白水凝胶(SH),并用其负载AuNR,得到具有光热治疗作用的复合水凝胶;通过紫外吸收光谱(UV)和扫描电子显微镜(SEM)等对水凝胶材料形貌及各项性能进行表征,并以3T3细胞为体外模型,对其生物相容性进行初步研究。结果表明:合成的金纳米棒吸收峰值在800 nm左右,对波长为808 nm的近红外激光具有良好的适配性;SH与AuNR@SH水凝胶均呈现多孔蜂窝状的形貌结构,并呈现出了良好的光热性能、力学性能和可降解性能;在体外细胞相容性实验中,AuNR@SH水凝胶展现出了良好的细胞相容性,具有作为光热试剂应用的潜力。

To solve the medical problem of the recurrence of tumors caused by residual cancer cells during surgical removal of tumors on the skin surface, natural polymer silk fibroin was used as the matrix material and coated with the photothermal agent gold nanorod(AuNR) to prepare composite hydrogel and conduct photothermal ablation on the residual cancer cells after the surgery to prevent the recurrence of tumor. Horse radish peroxidase(HRP) was used to cross-link the silk fibroin molecular chain to produce silk fibroin hydrogel(SH) to load AuNR and obtain composite hydrogel with a photothermal effect. Moreover, the morphologies and properties of the hydrogel were characterized by UV absorption spectrum(UV) and scanning electron microscopy(SEM), etc. Its biocompatibility was preliminarily studied, with 3 T3 cells as the in vitro model. The results indicated that the synthesized gold nanorod had an absorption peak at about 800 nm, and good adaptability to near-infrared laser with a wavelength of 808 nm. Both SH hydrogel and AuNR@SH hydrogel showed a porous cellular morphology, and presented excellent photothermal, mechanical, degradable properties. AuNR@SH hydrogel exhibited good cytocompatibility and had the potential to be used as a photothermal agent.