糖皮质激素对过敏性紫癜患儿肠道菌群的影响

Glucocorticoids on intestinal flora in children with Henoch-Schonlein purpura

目的探讨糖皮质激素(GC)对过敏性紫癜(HSP)患儿肠道菌群结构及多样性的影响。方法收集2017年9月至2019年3月住院治疗的HSP患儿60例的粪便标本,根据是否使用GC治疗分为GC组30例和无GC组30例,以21例健康儿童粪便样本为对照,采用试剂盒提取法提取样本DNA并应用Illumina HiSeq平台进行高通量测序,对测序结果进行生物学信息分析及统计学处理。结果基于分类操作单元(OTU)的物种丰度和多样性分析示,GC组和无GC组的OTU数、物种丰度估计参数CHAO1、多样性指数Shannon显著低于对照组(P均<0.01),多样性指数Simpson显著高于对照组(P<0.01)。GC组与无GC组的OTU、物种丰度估计参数ACE、CHAO1以及多样性指数Shannon、Simpson值比较差异无统计学意义(P均>0.05)。GC组、无GC组HSP患儿与健康儿童粪便菌群存在结构差异,在"科"分类学水平,GC组和无GC组以肠球菌科(42.0%、25.3%)、毛螺菌科(11.1%、13.8%)、双歧杆菌科(10.1%、15.8%)、瘤胃菌科(7.7%、9.0%)丰度较高;对照组以毛螺菌科(30.8%)、瘤胃菌科(24.6%)、拟杆菌科(14.4%)、双歧杆菌科(7.4%)有较高丰度。结论 HSP患儿肠道菌群物种丰度及多样性低于健康儿童;GC的使用可能与HSP患儿肠道菌群的相对丰度及多样性无关。

Objective To investigate the effects of glucocorticoids(GC) on the structure and diversity of intestinal flora in children with Henoch Schonlein purpura(HSP).Methods The stool samples of 60 HSP children from Sptember 2017 to March 2019 were collected and divided into GC group and non-GC group according to whether the children were treated with GC or not(n=30,each).The stool samples of 21 healthy children were taken as the control group.DNA extraction from samples was sequenced by Illumina HiSeq high-throughput sequencing platform, and the biological information analysis and statistical processing were performed on the sequenced data.Results The species abundance and diversity analysis based on the operational taxonomy unit(OTU) showed that the OTU number, species abundance estimation parameter CHAO1 and diversity Shannon index in GC group and non-GC group were significantly lower than those in control group(all P<0.01),while the diversity Simpson index was significantly higher than that in control group(P<0.01).There were no statistical differences in OUT,species richness estimation parameter ACE,CHAO1,Shannon and Simpson indexes between GC group and non-GC group(all P>0.05).The structure of fecal flora in children with HSP was different from that in healthy children.At the level of "family" taxonomy, Enterococcaceae(42.0%,25.3%),Lachnospiraceae(11.1%,13.8%),Bifidobacteriaceae(10.1%,15.8%),Ruminococcaceae(7.7%,9.0%) had a higher abundance in GC group and non-GC group;while Lachnospiraceae(30.8%),Ruminococcaceae(24.6%),Bacteroideae(14.4%),and Bifidobacteriaceae(7.4%) had a higher abundance in control group.Conclusion The species abundance and diversity of intestinal flora in HSP children are lower than those in healthy children, and use of glucocorticoids may not be associated with the relative abundance and diversity of intestinal flora in HSP children.