淫羊藿总黄酮和黄芪多糖配伍对骨质疏松大鼠的保护作用

Protective Effect of the Compatibility of Epimedium Flavonoids and Astragalus Polysaccharides on Osteoporosis Rats

目的:观察淫羊藿总黄酮和黄芪多糖配伍对去势骨质疏松大鼠的保护作用。方法:采用双侧卵巢切除术复制大鼠骨质疏松(OP)模型,将50只SD大鼠随机分为正常组、模型组、淫羊藿总黄酮组、黄芪多糖组和淫羊藿总黄酮+黄芪多糖组(各10只),分别给予相应药物灌胃,正常组和模型组给予等体积生理盐水。12周后处死大鼠,取血测定血钙(Ca)、血磷(P)、骨钙素(BGP),取左侧股骨,测定骨干质量、骨灰质量、骨Ca及骨P的含量。结果:淫羊藿总黄酮组、黄芪多糖组及淫羊藿总黄酮+黄芪多糖组均能改善血Ca、血P和BGP水平,增加骨干重、骨灰重、骨Ca及骨P的含量,差异有统计学意义(P<0.05);与单味药组相比,淫羊藿总黄酮+黄芪多糖组的作用尤为显著,差异有统计学意义(P<0.05)。结论:淫羊藿总黄酮和黄芪多糖配伍可有效改善去卵巢大鼠的骨质疏松,为补肾健脾法的临床应用提供了实验依据。

Objective:To study the protective effects of epimedium flavonoids and astragalus polysaccharides on osteoporosis rats.Methods:50 SD rats were randomly divided into normal group(n=10),model group(n=10),epimedium flavonoids group(n=10),astragalus polysaccharides group(n=10) and epimedium flavonoids + astragalus polysaccharides group(n=10).Each group was given corresponding drugs by gavage, and the normal group and model group were given equal volume of normal saline.After 12 weeks, all the rats were killed.The blood calcium(Ca),blood phosphorus(P) and Osteocalcin(BGP) were measured.The left femur was taken to measure the bone weight, bone ash weight, bone Ca and bone P content.Results:The levels of blood Ca, P and BGP were improved, and the contents of bone Ca, bone ash, bone Ca and bone P were increased in the group of epimedium flavonoids, astragalus polysaccharide and epimedium flavonoids + astragalus polysaccharides group(P<0.05).The effect of epimedium flavonoids + astragalus polysaccharide group was more significant than the single drug group(P<0.05).Conclusion:The combination of epimedium flavonoids and astragalus polysaccharides can effectively improve osteoporosis in ovariectomized rats, which provides experimental basis for the clinical application of tonifying the kidney and strengthening the spleen.