关于组织缺氧检测方法的研究进展

Research progress of the measurements of tissue hypoxia

肿瘤、炎症等疾病会造成氧供给的缺乏,一些炎性过程如正畸牙移动时牙周组织学反应也是缺血缺氧的过程,这将直接影响细胞的正常新陈代谢,严重的还会威胁生命。有效监测组织氧含量对于细胞组织进行正常生理活动非常重要。本文介绍几种评估氧含量的方法,包括:极谱氧电极测定、近红外线光谱成像、血氧水平依赖磁共振成像、缺氧相关标志物的检测、缺氧敏感性荧光探针等;每一种方法将从原理、特点、应用等着手,将各方法的优缺点进行横向的比较。

Many clinical diseases such as tumors and inflammation will cause the lack of oxygen supply, and some inflammatory processes such as orthodontic tooth movement is also a process of ischemia and hypoxia, which will directly affect the normal metabolism of cells, and will seriously threaten life. Effective monitoring is very important to maintain normal cell physiological activities. There are many methods that have been used to measure tissue hypoxia, including microelectrodes, near-infrared spectroscopy, blood oxygenation level dependent magnetic resonance imaging, molecules induced by hypoxia and hypoxia sensitive fluorescent probe. In this paper, we reviewed the technologies, characteristics, applications, limitations and precautions of each approach, and made a horizontal comparison of the advantages and disadvantages of each method.