白藜芦醇抗切除卵巢大鼠发生骨质疏松的作用研究

Effect of resveratrol on the development of osteoporosis in ovariectomized rats

目的研究白藜芦醇(resveratrol,R)抗切除卵巢大鼠发生骨质疏松的作用。方法6月龄Wistar雌性大鼠40只,随机数字表法分为假手术组(Sham)、去卵巢组(OVX)、淫羊藿苷组(I)和白藜芦醇组(R)。I组给予25 mg/(kg·d)的淫羊藿苷灌胃,R组给予8.4 mg/(kg·d)的白藜芦醇灌胃,Sham和OVX组灌胃等体积蒸馏水。根据体质量每两周进行一次灌胃剂量调整。8周后待各组间骨密度出现显著性差异后立即处死,统计大鼠体质量并计算大鼠器官指数,检测离体骨密度、骨生物力学、血清生化指标,观察骨组织形态并测量分析骨小梁相关静态参数。结果各组大鼠体质量并未出现统计学差异,除子宫系数外其他器官指数无统计学差异(P>0.05),但OVX组的子宫指数显著低于Sham组(P<0.05),I组和R组子宫指数显著高于OVX组(P<0.05);与OVX组相比,I组和R组的骨密度、最大载荷、弹性模量、骨形成指标OC以及Tb.N、BV/TV均显著升高(P<0.05),而骨吸收指标TRACP-5b含量和Tb.SP均显著降低(P<0.01);骨组织形态观察结果也显示I组和R组骨小梁网状结构呈致密性增加,骨小梁数目明显变多。R组与I组相比,其各指标平均值略低于I组,差异均无统计学意义(P>0.05)。结论白藜芦醇可能通过增加骨密度、提高骨强度、促进骨形成、抑制骨吸收、改善骨质量从而发挥抗骨质疏松的作用。

Objective To study the effect of resveratrol(R)on osteoporosis in ovariectomized rats.Methods Forty 6-month-old Wistar female rats were randomly divided into sham group(Sham),ovariectomy group(OVX),icariin group(I),and resveratrol group(R).Rats in group I received 25 mg/(kg·d)of icariin by gavage.Rats in group R received 8.4 mg/(kg·d)resveratrol by gavage.Rats in Sham and OVX group received equal volume of distilled water.According to body weight,the gavage dose was adjusted every 2 weeks.The rats were killed 8 weeks later when there was a significant difference in bone mineral density(BMD)between the groups.The body weight of the rats was counted and the organ index of the rats was calculated.The in vitro BMD,bone biomechanics,serum biochemical indicators,bone tissue morphology,and static parameters related to trabecular bone were measured and analyzed.Results There was no statistical difference in the body weight of rats between each group,and there was no statistical difference in the index of other organs except for the uterine coefficient(P>0.05).The uterine index of OVX group was significantly lower than that of sham group(P<0.05).The uterine index of group I and R was significantly higher than that of OVX group(P<0.05).Compared with those in OVX group,BMD,maximum load,elastic modulus,and bone formation index OC,TB.N,and BV/TV in group I and R increased significantly(P<0.05),while the content of bone resorption indexes Tracp5b and TB.SP decreased significantly(P<0.01).The observation result of bone morphology showed that the trabecular reticular structure in group I and group R increased in density,and the number of trabeculae increased significantly.The average value of each index in Group R was slightly lower than that in group I,with no significant difference(P>0.05).Conclusion Resveratrol may play an anti-osteoporotic effect by increasing BMD,improving bone strength,promoting bone formation,inhibiting bone resorption,and improving bone quality.