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敲低程序性死亡配体1抑制人乳腺癌细胞生长与转移

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摘要 目的分析程序性死亡配体1(PDL1)在人乳腺癌MDA-MB-231细胞的生物学功能。方法 qRTPCR检测人乳腺癌细胞系(MDA-MB-231和HCC38)与人正常乳腺细胞系(MCF-10A)中PDL1的表达水平;运用MTT实验检测si-PDL1对人乳腺癌MDA-MB-231细胞的生长能力的作用;划痕实验检测si-PDL1对人乳腺癌MDA-MB-231细胞的迁移能力的作用;Transwell侵袭实验检测si-PDL1对人乳腺癌MDA-MB-231细胞的侵袭作用。结果 qRT-PCR检测结果显示,人乳腺癌细胞系(MDA-MB-231和HCC38)中PDL1的表达水平明显高于人正常乳腺细胞系(MCF-10A)的表达水平(P <0.01);MTT检测发现,干扰PDL1可抑制人乳腺癌MDA-MB-231细胞的生长(P <0.05);划痕实验检测发现,干扰PDL1可抑制人乳腺癌MDA-MB-231细胞的迁移能力(P <0.05);Transwell侵袭实验显示,干扰PDL1可抑制人乳腺癌MDA-MB-231细胞的侵袭(P <0.05)。结论干扰PDL1可抑制人乳腺癌MDA-MB-231细胞的生长与迁移侵袭。
出处 《临床合理用药杂志》 2021年第11期176-177,共2页 Chinese Journal of Clinical Rational Drug Use
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