草莓叶水提物对UVB致皮肤损伤的保护作用

Protective effect of strawberry(Fragaria × Ananassa) leaves water extract on skin damage by UVB

为了评价草莓叶水提物(SLWE)延缓皮肤衰老功效,对SLWE成分、抗氧化活性、抑制非酶糖基化(NEG)能力进行了测定;探讨了SLWE对人包皮成纤维细胞(HFF-1)细胞活率、HFF-1中透明质酸(HA)、人Ⅰ型胶原蛋白(COLⅠ)、羟脯氨酸(HYP)、人基质金属蛋白酶1(MMP-1)、人基质金属蛋白酶9(MMP-9)含量的影响。在中波紫外线(UVB)致HFF-1损伤模型基础上,考察了SLWE对保护组活性氧(ROS)、丙二醛(MDA)、MMP-1和超氧化物歧化酶(SOD)的影响,探讨SLWE对UVB致HFF-1损伤保护机制。结果表明,SLWE富含VC、槲皮素、绿原酸、没食子酸及儿茶素类物质,其中,槲皮素含量12.86 mg/L,表儿茶素含量10.13 mg/L。SLWE自由基清除能力良好,清除不同自由基能力依次为O2–·>DPPH·>ABTS·>亚铁离子螯合>·OH;体积分数为1%的SLWE水溶液对NEG的抑制率为87.17%;体积分数大于3%的SLWE水溶液可提高HFF-1中HA、COLⅠ和HYP含量(p<0.05),体积分数为6%的SLWE水溶液可降低MMP-1含量(p<0.01),表明SLWE具有提升皮肤含水量,增加胶原含量,抑制胶原降解,实现延缓衰老的功效;SLWE可有效降低因UVB刺激而产生的ROS,体积分数6%的SLWE水溶液可显著降低MDA含量,抑制MMP-1表达,提高SOD活力,说明SLWE有助于缓解氧化应激造成的细胞损伤。

In order to evaluate the anti-aging effect of strawberry(Fragaria × Ananassa) leaves water extract(SLWE), the composition, antioxidant activity and non-enzymatic glycation(NEG) inhibition rate of SLWE were measured. The effects of SLWE on cell viability and contents of hyaluronic acid(HA), human collagen type Ⅰ(COL Ⅰ), hydroxyproline(HYP), matrix metalloproteinase 1(MMP-1) and matrix metalloproteinase 9(MMP-9) of human foreskin fibroblasts(HFF-1) were discussed. Based on the UVB induced damage model of HFF-1, the effects of SLWE on reactive oxygen species(ROS), malondialdehyde(MDA), MMP-1 and superoxide dismutase(SOD) in the protection group were investigated, and the protective mechanism of SLWE on UVB-induced HFF-1 damage was explored. The results showed that SLWE was rich in VC, quercetin, chlorogenic acid, gallic acid and catechins, among which, quercetin and epicatechin contents were 12.86 mg/L and 10.13 mg/L respectively. SLWE had excellent free radical scavenging ability, the scavenging abilities of different free radicals were in order, O2–· >DPPH· >ABTS· > ferrous chelation > ·OH. The NEG inhibition rate of volume fraction 1% SLWE aqueous solution was 87.17%. More than volume fraction 3% SLWE aqueous solution could improve contents of HA, COLⅠand HYP(p< 0.05) in HFF-1, and volume fraction 6% SLWE could decrease content of MMP-1(p< 0.01), suggesting that SLWE could skin moisture content, increase collagen content, inhibit collagen degradation, and realize the effect of delaying aging. SLWE could effectively reduce ROS generated by UVB stimulation, volume fraction 6% SLWE aqueous solution could significantly reduce MDA dosage, inhibit MMP-1 expression, and improve activity of SOD, which indicated that SLWE contributed to alleviating cell damage caused by oxidative stress.