明胶中总铅、总铬标准物质的研制

Preparation for reference material of total lead and total chromium in gelatin

目的建立食用明胶中总铅和总铬标准物质的研制和定值方法。方法以食用明胶为研究基体,研制了明胶中总铅、总铬成分分析标准物质。经过目标物基体添加、熔融混匀、冷却、干燥、灭菌等特殊工艺过程制备并分装。采用F-检验评价标准物质的均匀性,采用t检验(t-test)评价标准物质的长期和短期稳定性,采用10家权威实验室多种测量方法联合定值的方式赋值,并对定值结果进行了不确定度评估。结果明胶中总铅、总铬标准物质定值结果为:总铅(32.2±1.6) mg/kg, k=2;总铬(43.0±2.2) mg/kg, k=2。结论该标准物质对持续填补我国明胶中总铅、总铬成分分析标准物质起到重要作用。

Objective To establish the preparation and certification method of total lead and total chromium standard substance in edible gelatin. Methods The edible gelatin was used as the research matrix, and the analytical standard substances for total lead and total chromium in gelatin were developed, and the preparation and subpackaging was through special proces such as adding a target substrate, melting and mixing, cool, drying, sterilizing and that like. The F-test was used to evaluate the homogeneity of the reference substance, and the t-test was used to evaluate the long-term and short-term stability of the reference substance. A combination of multiple measurement methods and setting method from 10 authoritative laboratories was used for assignment, and the uncertainty of the setting result was evaluated. Results The certified values of total lead and total chromium in gelatin were as follows: total lead(32.2±1.6) mg/kg, k=2;total chromium(43.0±2.2) mg/kg, k=2. Conclusion The standard substance plays an important role in continuously filling that analysis standard substance of the total lead and the total chromium in the gelatin in China.