升陷汤及单味药材水提物对缺氧/复氧致心肌损伤的保护作用

Protective effect of Shengxian decoction and the decoction of single herb component against myocardial injury induced by hypoxia/reoxygenation

目的通过观察升陷汤及单味药材水提物对离体培养的大鼠心肌细胞缺氧/复氧损伤的影响,并对其作用机制进行初步探讨。方法培养H9C2大鼠心肌细胞,共分成8组:空白对照组,缺氧/复氧组(模型组),缺氧复氧损伤后药物干预组(升陷汤全方及5个单味药材水提物组)。分别对心肌细胞凋亡率、心肌细胞的活力、细胞内活性氧(ROS)活性、细胞内钙离子浓度(Ca^(2+))等指标进行检测。结果升陷汤全方及黄芪、知母等药材干预能明显降低细胞凋亡率、细胞内ROS活性和Ca^(2+)浓度(P<0.05),其中,全方的作用最强。与缺氧/复氧组细胞内ROS活性和Ca^(2+)浓度增加至空白对照组的2.49倍及1.71倍相比,全方能使细胞内ROS活性和Ca^(2+)浓度增加率降至缺氧/复氧组的41.37%和15.20%。结论升陷汤及单味药材对缺氧/复氧致心肌损伤具有保护作用,该作用的机制可能通过抑制细胞凋亡、降低细胞内ROS以及Ca^(2+)的浓度所致。

Objective To study the protective effect of Shengxian decoction and the single herb decoction against myocardial injury induced by hypoxia/reoxygenation.Methods The H9c2 cells were cultured to establish hypoxia/reoxygenation model.Rats were divided into 8 groups:normal control group,hypoxia/reoxygenation group(model group)and treated groups(Shengxian decoction and the single herb decoction).The apoptotic rate of cardiomyocytes,the activity of reactive oxygen species(ROS)and intracellular calcium concentration(Ca^(2+))were measured.Results Compared with hypoxia/reoxygenation group,the apoptosis rate,ROS activity and intracellular Ca^(2+)concentration were significantly lower in all treated groups(P<0.05).The ROS activity and intracellular Ca^(2+)concentration was decreased by 41.37%and 15.20%in Shengxian decoction group compared to the model group.Conclusion Shengxian decoction and the single herb decoction had protective effect on myocardial injury induced by hypoxia/reoxygenation.