基于代谢组学的食管鳞癌代谢特征与临床意义研究

Metabonomics-based Study of Metabolic Characteristics and Clinical Significance for Esophageal Squamous Cell Carcinoma

【目的】比较食管鳞癌患者和健康志愿者的血清代谢谱,寻找食管鳞癌特有的代谢标志物。【方法】选择一组明确诊断的食管鳞癌患者作为实验组、一组健康志愿者作为对照组,以液相色谱-质谱法(LC-MS)检测其血清轮廓代谢谱,通过单变量分析和多变量(主成分分析及偏最小二乘回归分析法等)比较两组代谢谱并寻找差异性代谢物,通过代谢物质通路的富集分析探讨其可能异常生物学过程。【结果】入组食管鳞癌患者20例为实验组,健康对照20例为对照组,变异倍数分析和T检验等单因素分析结果得出21种差异性代谢物(P<0.05);选择单变量分析P<0.05、变异倍数(FC)>2、变量投影重要度(VIP)>1,通过偏最小二乘回归的多变量统计分析,最终得出差异性代谢物为肌苷、苯丙氨酸二肽、羟基吲哚硫酸、2-顺式二十烯酸)、硫酸普萘诺酮)、δ-戊丙酰胺);富集分析显示上述代谢物通路中均有不同程度的能量代谢异常,且为过度消耗状态。【结论】食管鳞癌和正常对照组之间的血清代谢谱存在明显差异,主要表现在能量代谢上的过度消耗。

【Objective】To compare the serum metabolic profiles of patients with esophageal squamous cell carcinoma and healthy volunteers,and to find metabolite markers of esophageal squamous cell carcinoma.【Methods】Select a group of patients with esophageal squamous cell carcinoma as the experimental group and a group of health volunteers served as a control group,and their serum profile metabolic profiles were detected by liquid chromatography-mass spectrometry(LCMS).Variable analysis and multivariate(principal component analysis and partial least square regression analysis).Differential metabolites were discovered by comparing the metabolic profiles of the two groups.Its possible abnormal biological processes were explored through enrichment analysis of metabolite pathways.【Results】Twenty patients with esophageal squamous cell carcinoma were included in the experimental group,and 20 healthy controls were the control group.The multiples of variation;The results of Univariate analysis detected 21 different metabolites(P<0.05);The differential metabolites were finally Inosine,Phe Phe,Indoxylsulfuric acid,2-cis Eicosenoic Acid,Pregnanolone sulfate,delta-Valerolactam through multivariate statistics through partial least squares regression;Enrichment analysis showed that the above mentioned metabolite pathways all have different degrees of abnormal energy metabolism,and they were in a state of excessive consumption.【Conclusion】There is a significant difference in the serum metabolic profile between esophageal squamous cell carcinoma and the normal control group,which is mainly manifested in the excessive consumption of energy metabolism.