摘要
[目的]为了分析广西牛超数排卵技术实施现状,[方法]试验设计在广西宾阳、都安、大化、崇左等4个肉牛养殖场(4组)各选择16头西门塔尔成年母牛作为试验母牛,采用孕酮栓(GIOB)+促卵泡素(FSH)+氯前列烯醇(PGF2)的方法对试验母牛进行超数排卵,观测试验母牛获得总胚胎数、可用胚数、一级胚数,分析超数排卵效果。[结果]结果显示,超数排卵处理后,试验牛每头获胚胎3~14枚,平均(8.02±2.75)枚;每头获可用胚1~9枚,平均(4.64±1.84)枚;每头获一级胚0~3枚,平均(1.56±0.94)枚,总胚、可用胚、一级胚组间均无显著差异(P>0.05),但是,都安组头均总胚、可用胚、一级胚比所有试验牛平均值分别提高5.99%,6.47%,16.03%。[结论]结果表明,广西规模化牛场实施超数排卵进展顺利,超数排卵效果良好,组间无显著差异(P>0.05),都安组在获得胚胎数量和质量方面最佳。
[Objective]In order to analyze the current implementation situation on superovulation technology in Guangxi.[Method]According to the experimental design,16 Simmental adult cows were selected in Guangxi Binyang,Duan,Dahua and Chongzuo beef cattle farms,respectively.The progesterone plug(GIOB)+follicle-stimulating hormone(FSH)+chlorine forefront enol(PGF2)were used for superovulation,and the total number of embryos,the number of available embryos,embryo number were observed to evaluate the superovulation effect.[Results]The results showed that after the treatment of superovulation,3-14 embryos were obtained from each cow,with an average of 8.02±2.75 embryos.1-9 embryos were available per head,with an average of 4.64±1.84 embryos.0~3 first class embryos were obtained from each head,with an average of 1.56±0.94.There was no significant difference between total embryos,available embryos and first class embryos(P>0.05).However,the average total embryos,available embryos and first class embryos of Duan group increased 5.99%,6.47%and 16.03%compared with the average of all the experimental cattle.[Conclusion]The results showed that the implementation of superovulation in large-scale cattle farms in Guangxi progressed smoothly,and the superovulation effect was good,and there was no significant difference between groups(P>0.05).Duan group was the best in terms of the number and quality of embryos obtained.
作者
文信旺
贾银海
潘锐
吴思谦
黄香
戴黄山
黄明光
梁金逢
周晓情
王启芝
WEN Xin-wang;JIA Yin-hai;PAN Rui;WU Si-qian;HUANG Xiang;DAI Huang-shan;HUANG Ming-guang;LIANG Jin-feng;ZHOU Xiao-qing;WANG Qi-zhi(Institute of Animal Husbandry of Guangxi Zhuang Autonomous Region,Nanning 530001;Key Laboratory of Livestock Genetic Improvement of Guangxi,Nanning 530001;Aquatic Animal Husbandry and Veterinary Station in Banlan Town of Rong’an County,Rong’an,Guangxi 545400)
出处
《中国牛业科学》
2021年第2期13-16,共4页
China Cattle Science
基金
广西科技重大专项
广西创新驱动发展专项资金项目“广西良种肉牛繁育关键技术与产业化应用示范”(桂科AA17204028)
广西农业科技自筹经费项目“肉牛生态养殖关键技术创新及推广应用”(Z201920)
广西壮族自治区畜牧研究所,广西家畜遗传改良重点实验室资金支持项目。
关键词
西门塔尔母牛
超数排卵
总胚
可用胚
超数排卵效果
Simmental cow
superovulation
total number of embryo
available embryo
superovulation effect