本氏烟中与番茄斑萎病毒N蛋白互作的寄主因子的筛选

Host Factors in Tobacco Interacting with N Protein of Tomato Spotted Wilt Virus

【目的】番茄斑萎病毒(Tomato spotted wilt virus,TSWV)是植物多分体负义链RNA病毒的代表性成员。TSWV的N蛋白在病毒感染的寄主植物内表达量高,可能调控病毒对寄主的感病,探究N蛋白通过影响哪些寄主蛋白的表达来完成病毒的侵染,以期为深入解析寄主蛋白调控病毒的分子机制提供理论基础,为后续有效的防控TSWV提供新的思路。【方法】以TSWV N蛋白作为诱饵蛋白,采用酵母双杂交(Yeast two-hybrid,Y2H)的方法筛选本氏烟内与TSWV N相互作用的寄主蛋白。【结果】共筛选获得15种与TSWV N相互作用的寄主蛋白。【结论】这些介体因子主要参与色素的生物合成、类囊体膜的组装、植物防御反应和核糖体生物发生的过程,调节脂质代谢和细胞功能,可能是翻译调控的辅助蛋白,在植物发育和对非生物胁迫的反应中发挥着重要作用。

【Objective】Host factors in Nicotiana benthamiana that interact with the N protein of tomato spotted wilt virus(TSWV),a representative member of plant negative stranded RNA viruses,were identified in preparation for further analysis on the regulation mechanism and in search for effective prevention and control of TSWV-induced disease on plants.【Method】Using the yeast two-hybrid(Y2H)method,proteins in N.benthamiana that interacted with the bait N protein of TSWV were screened.【Result】Fifteen host proteins were identified.【Conclusion】These identified proteins are known to be associated with pigment biosynthesis,thylakoid membrane assembly,plant defense response,ribosome biogenesis,lipid metabolism,and cellular functions in plants.Being upregulated in N.benthamiana infected by TSWV,these proteins might also act as auxiliary proteins in translating the regulation playing an important role in the development and response to abiotic stress of the plant.