广东省深圳地区类风湿关节炎患者血清25-(OH)VitD3水平及其受体基因rs2228570 T/C位点多态性的相关性研究

Correlation Study of Serum 25-(OH)VitD3 Level and the Polymorphism of Its rs2228570 T/C in Patients with Rheumatoid Arthritis in Shenzhen

目的了解深圳地区类风湿关节炎(rheumatoid arthritis,RA)患者25-羟基维生素D3[25-(OH)VitD3]水平及维生素D受体(VDR)基因rs2228570 T/C位点多态性,并探讨其与RA发病之间的相关性。方法选择126例RA患者作为RA组,另选取120例健康体检者作为对照组。分别检测血清25-(OH)VitD3水平及VDR基因rs2228570T/C位点多态性。结果RA组血清25-(OH)VitD3水平(31.57±9.36nmol/L)明显低于对照组(65.92±14.57nmol/L),两者差异有统计学意义(t=9.1542,P=0.0137),其中RA组男性(42.16±11.75nmol/L)明显高于女性(27.50±8.12nmol/L),两者差异有统计学意义(t=6.1374,P=0.0209),而对照组男性(63.95±13.80nmol/L)略低于女性(66.58±15.09nmol/L),两者差异无统计学意义(t=0.9452,P=0.1295)。RA组高度活动组25-(OH)VitD3水平(19.37±5.82nmol/L)明显低于中度活动组(30.69±8.63nmol/L),而中度活动组明显低于低活动组(38.32±10.78nmol/L),不同组别间差异有统计学意义(F=17.9351,P=0.0082)。RA组VDR基因rs2228570 T/C位点CC基因型和C等位基因检出率(41.27%和54.76%)明显高于对照组(17.50%和32.08%),两者差异有统计学意义(χ^(2)=6.0547,4.9752,均P<0.05)。RA组VDR基因rs2228570 T/C位点CC基因型和C等位基因检出率男性(28.57%和44.29%)明显低于女性(46.15%和58.79%),两者差异有统计学意义(χ^(2)=3.9728,3.1086,均P<0.05)。RA组CC基因型血清25-(OH)VitD3水平(18.95±4.59nmol/L)明显低于TC和TT基因型(38.62±11.03nmol/L和41.98±11.92nmol/L),差异有统计学意义(t=5.0164~5.8126,均P<0.05),而TC和TT基因型之间差异无统计学意义(t=1.2068,P=0.1178)。结论广东深圳地区RA患者25-(OH)VitD3水平明显降低,且VDR基因rs2228570T/C位点存在多态性分布,与25-(OH)VitD3水平之间存在一定关系,其中CC基因型可能是该地区RA发病的危险易感基因之一。

Objective To investigate the level of 25-hydroxyvitamin D3[25-(OH)VitD3]and the polymorphism of the vitamin D receptor(VDR)gene RS2228570 T/C,and explore the correlation between it and the incidence of rheumatoid arthritis(RA)in patients with rheumatoid arthritis(RA)in Shenzhen.Methods 126 patients with RA were selected as the RA group,and 120 healthy subjects were selected as the control group.The level of 25-(OH)VitD3 in serum and polymorphisms of rs2228570T/C site of VDR gene were detected.Results The level of 25-(OH)VitD3 in RA group(31.57±9.36nmol/L)was significantly lower than control group(65.92±14.57nmol/L),the difference was statistically significant(t=9.1542,P=0.0137).In RA group,males(42.16±11.75nmol/L)were significantly higher than females(27.50±8.12nmol/L),the difference was statistically significant(t=6.1374,P=0.0209).In control group,males(63.95±13.80nmol/L)were slightly lower than females(66.58±15.09nmol/L),with no statistically significant difference(t=0.9452,P=0.1295).The level of 25-(OH)VitD3 in the highly active RA group(19.37±5.82nmol/L)was significantly lower than moderately active RA group(30.69±8.63nmol/L),while the moderately active RA group was significantly lower than the low-active RA group(38.32±10.78nmol/L),with statistically significant differences between the different groups(F=17.9351,P=0.0082).The detection rate of VDR RS2228570 T/C site CC genotype and C allele in RA group(41.27% and 54.76%)was significantly higher than control group(17.50% and 32.08%),and the difference was statistically significant(χ^(2)=6.0547,4.9752,all P<0.05).The detection rate of CC genotype and C allele of VDR RS2228570 T/C site CC in RA group males(28.57% and 44.29%)was significantly lower than females(46.15%and 58.79%),and the difference was statistically significant(χ^(2)=3.9728,3.1086,all P<0.05).In RA group,the level of 25-(OH)VitD3 of CC genotype(18.95±4.59nmol/L)was significantly lower than TC and TT genotypes(38.62±11.03nmol/L and 41.98±11.92nmol/L),and the difference was statistically significant(t=5.0164~5.8126,all P<0.05),while the difference between TC and TT genotypes was not statistically significant(t=1.2068,P=0.1178).Conclusion The level of 25-(OH)VitD3 was significantly decreased in RA patients in Shenzhen,Guangdong,and there was a polymorphic distribution of VDR gene rs2228570T/C,which was related to the level of 25-(OH)VitD3 to a certain extent.CC genotype may be one of the risk susceptibility genes for RA in this area.